Journal of Applied Physiology AJP: Heart and Circulatory Physiology
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J Appl Physiol 85: 1806-1812, 1998;
8750-7587/98 $5.00
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Vol. 85, Issue 5, 1806-1812, November 1998

Quantitation of intracellular [Na+] in vivo by using TmDOTP5minus  as an NMR shift reagent and extracellular marker

Patrick M. Winter3, Viswanathan Seshan1, Janice D. Makos2, A. Dean Sherry1,2, Craig R. Malloy1,4, and Navin Bansal1

1 Department of Radiology, The Mary Nell and Ralph B. Rogers Magnetic Resonance Center, University of Texas Southwestern Medical Center, Dallas, 75235-9085 2 Department of Chemistry, University of Texas at Dallas, Richardson, 75083-0688; 3 Biomedical Engineering Program, University of Texas at Arlington, Arlington 76019; and 4 Dallas Veterans Affairs Medical Center, Dallas, Texas 75216

A method is presented to measure the absolute concentration of intracellular Na+ ([Na+]i) in vivo by using interleaved 23Na- and 31P-nuclear magnetic resonance (NMR) spectroscopy and TmDOTP5- as shift reagent and chemical marker of tissue extracellular space (ECS). The technique was used to determine [Na+]i and relative ECS in livers of control rats (21 ± 3 and 0.11 ± 0.02 mM, respectively) and in rats exposed to carbon tetrachloride (103 ± 29 and 0.23 ± 0.03 mM, respectively). The NMR measurements were confirmed independently on excised tissue samples by using atomic absorption spectroscopy. The results confirm that TmDOTP5- can be used as a combined cation shift reagent and ECS marker, thereby allowing quantitation of [Na+]i in vivo by NMR.

nuclear magnetic resonance; sodium; liver; atomic absorption spectrometry


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