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Division of Physiology, Department of Medicine, University of California, San Diego, La Jolla, California 92093
There is
evidence that the concentration of the high-energy phosphate
metabolites may be altered during steady-state submaximal exercise
by the breathing of different fractions of inspired
O2 (FIO2). Whereas
it has been suggested that these changes may be the result of
differences in time taken to achieve steady-state O2 uptake
(
O2) at different
FIO2 values, we postulated that they are due to a direct effect of
O2 tension. We used
31P-magnetic resonance
spectroscopy during constant-load, steady-state submaximal exercise to
determine 1) whether changes in
high-energy phosphates do occur at the same
O2 with varied
FIO2 and
2) that these changes are not due to
differences in O2
onset kinetics. Six male subjects performed steady-state submaximal plantar flexion exercise [7.2 ± 0.6 (SE) W] for 10 min
while lying supine in a 1.5-T clinical scanner. Magnetic resonance
spectroscopy data were collected continuously for 2 min before
exercise, 10 min during exercise, and 6 min during recovery. Subjects
performed three different exercise bouts at constant load with the
FIO2 switched after 5 min of
the 10-min exercise bout. The three exercise treatments were
1)
FIO2 of 0.1 switched to
0.21, 2)
FIO2 of 0.1 switched to
1.00, and 3)
FIO2 of 1.00 switched to
0.1. For all three treatments, the
FIO2 switch significantly (P 
0.05) altered phosphocreatine:
1) 55.5 ± 4.8 to 67.8 ± 4.9% (%rest); 2) 59.0 ± 4.3 to
72.3 ± 5.1%; and 3) 72.6 ± 3.1 to 64.2 ± 3.4%, respectively. There were no significant
differences in intracellular pH for the three treatments. The results
demonstrate that the differences in phosphocreatine concentration with
varied FIO2 are not the
result of different O2
onset kinetics, as this was eliminated by the experimental design.
These data also demonstrate that changes in intracellular oxygenation,
at the same work intensity, result in significant changes in cell homeostasis and thereby suggest a role for metabolic control by O2 even during submaximal
exercise.
fraction of inspired oxygen; skeletal muscle; intracellular oxygenation
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