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O2 on-kinetics
in isolated in situ canine muscle
Department of Medicine, University of California, San Diego, La Jolla, California 92093-0623; Istituto di Tecnologie Biomediche Avanzate, Consiglio Nazionale delle Ricerche, I-20090 Segrate (MI), Italy; and Department of Health and Human Performance, Auburn University, Auburn, Alabama 36849-5323
To test the
hypothesis that muscle O2 uptake
(
O2) on-kinetics is
limited, at least in part, by peripheral
O2 diffusion, we determined the
O2 on-kinetics in
1) normoxia (Control);
2) hyperoxic gas breathing
(Hyperoxia); and 3) hyperoxia and
the administration of a drug (RSR-13, Allos Therapeutics), which
right-shifts the Hb-O2
dissociation curve (Hyperoxia+RSR-13). The study was conducted in
isolated canine gastrocnemius muscles
(n = 5) during transitions from rest
to 3 min of electrically stimulated isometric tetanic contractions
(200-ms trains, 50 Hz; 1 contraction/2 s; 60-70% peak
O2). In all conditions,
before and during contractions, muscle was pump perfused with
constantly elevated blood flow (
), at a level
measured at steady state during contractions in preliminary trials with
spontaneous . Adenosine was infused
intra-arterially to prevent inordinate pressure increases with the
elevated . was measured
continuously, arterial and popliteal venous
O2 concentrations were determined
at rest and at 5- to 7-s intervals during contractions, and
O2 was calculated as
· arteriovenous O2 content difference.
PO2 at 50%
HbO2
saturation (P50) was calculated.
Mean capillary PO2
(
cO2)
was estimated by numerical integration.
P50 was higher in Hyperoxia+RSR-13
[40 ± 1 (SE) Torr] than in Control and in Hyperoxia (31 ± 1 Torr). After 15 s of contractions,
cO2
was higher in Hyperoxia (97 ± 9 Torr) vs. Control (53 ± 3 Torr) and in Hyperoxia+RSR-13 (197 ± 39 Torr) vs. Hyperoxia. The
time to reach 63% of the difference between baseline and steady-state
O2 during contractions was 24.7 ± 2.7 s in Control, 26.3 ± 0.8 s in Hyperoxia, and 24.7 ± 1.1 s in Hyperoxia+RSR-13 (not significant). Enhancement of
peripheral O2 diffusion (obtained
by increased
cO2
at constant O2 delivery) during
the rest-to-contraction (60-70% of peak
O2) transition did not
affect muscle O2
on-kinetics.
gas exchange kinetics; submaximal exercise; muscle oxidative metabolism
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