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1 Laboratoire de Recherche en Pathologie Infectieuse and 3 Laboratoire de Biophysique, Faculté de Médecine de Lille, 59045 Lille, France; and 2 Department of Medicine, Louisiana State University Medical Center, New Orleans, Louisiana 70112
Several methodologies have been developed to assess
alveolocapillary membrane permeability in acute lung injury. The
purpose of this study was to determine the reliability of FITC-dextran compared with radioactive tracers to assess lung permeability alterations. After intraperitoneal administration of
-naphthylthiourea (ANTU, 50 mg/kg) or DMSO-ANTU vehicle, the animals
were euthanized and their lungs were studied in an isolated-lung
preparation. FITC-dextran or radiolabeled tracers were added to the
perfusate. At 2 h the bronchoalveolar lavage (BAL) fluid from the ANTU
group showed a significantly greater amount of fluorescence in the
supernatant after centrifugation of BAL fluid compared with the DMSO
group. Consistent results were observed with the radioactive tracers: there was an increase in extravascular albumin space and extravascular lung water compared with the control group. No cleavage of the FITC
from the dextran molecule was evident by chromatography comparing samples recovered from the BAL fluid to the pure FITC-dextran molecule.
In conclusion, measurement of FITC-dextran in the supernatant of BAL
fluid after intravascular administration is a reliable method of
assessing lung permeability changes in vivo and ex vivo.
bronchoalveolar lavage; -naphthylthiourea; permeability; lung
injury; dextran
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