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Copenhagen Muscle Research Centre, Rigshospitalet, DK-2200 Copenhagen N, Denmark
The present study compared the microdialysis ethanol
outflow-inflow technique for estimating blood flow (BF) in skeletal
muscle of humans with measurements by Doppler ultrasound of femoral
artery inflow to the limb
(BFFA). The microdialysis probes
were inserted in the vastus lateralis muscle and perfused with a Ringer
acetate solution containing ethanol,
[2-3H]adenosine (Ado),
and
D-[14C(U)]glucose.
BFFA at rest increased from
0.16 ± 0.02 to 1.80 ± 0.26 and 4.86 ± 0.53 l/min
with femoral artery infusion of Ado (AdoFA,i) at 125 and 1,000 µg · min
1 · l1
thigh volume (low dose and high dose, respectively;
P < 0.05) and to 3.79 ± 0.37 and
6.13 ± 0.65 l/min during one-legged, dynamic, thigh muscle exercise
without and with high AdoFA,i,
respectively (P < 0.05). The ethanol
outflow-to-inflow ratio (38.3 ± 2.3%) and the probe recoveries
(PR) for [2-3H]Ado
(35.4 ± 1.6%) and for
D-[14C(U)]glucose
(15.9 ± 1.1%) did not change with
AdoFA,i at rest (P = not significant). During exercise
without and with AdoFA,i, the
ethanol outflow-to-inflow ratio decreased
(P < 0.05) to a similar level of
17.5 ± 3.4 and 20.6 ± 3.2%, respectively
(P = not significant), respectively,
while the PR increased (P < 0.05) to
a similar level (P = not significant)
of 55.8 ± 2.8 and 61.2 ± 2.5% for
[2-3H]Ado and to 42.8 ± 3.9 and 45.2 ± 5.1% for
D-[14C(U)]glucose.
Whereas the ethanol outflow-to-inflow ratio and PR correlated inversely
and positively, respectively, to the changes in BF during muscular
contractions, neither of the ratio nor PR correlated to
the AdoFA,i-induced BF increase.
Thus the ethanol outflow-to-inflow ratio does not represent skeletal
muscle BF but rather contraction-induced changes in molecular transport in the interstitium or over the microdialysis membrane.
adenosine; exercise; heterogeneity; hyperemia ; glucose
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