Journal of Applied Physiology Information on EB 2010
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

J Appl Physiol 85: 627-634, 1998;
8750-7587/98 $5.00
This Article
Right arrow Full Text Free
Right arrow Full Text (PDF) Free
Right arrow Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Richardson, R. S.
Right arrow Articles by Wagner, P. D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Richardson, R. S.
Right arrow Articles by Wagner, P. D.
Vol. 85, Issue 2, 627-634, August 1998

Lactate efflux from exercising human skeletal muscle: role of intracellular PO2

Russell S. Richardson1, Elizabeth A. Noyszewski2, John S. Leigh2, and Peter D. Wagner1

1 Department of Medicine, University of California, San Diego, La Jolla, California 92093-0623; and 2 Department of Radiology, University of Pennsylvania, Philadelphia, Pennsylvania 19104-6021

It remains controversial whether lactate formation during progressive dynamic exercise from submaximal to maximal effort is due to muscle hypoxia. To study this question, we used direct measures of arterial and femoral venous lactate concentration, a thermodilution blood flow technique, phosphorus magnetic resonance spectroscopy (MRS), and myoglobin (Mb) saturation measured by 1H nuclear MRS in six trained subjects performing single-leg quadriceps exercise. We calculated net lactate efflux from the muscle and intracellular PO2 with subjects breathing room air and 12% O2. Data were obtained at 50, 75, 90, and 100% of quadriceps maximal O2 consumption at each fraction of inspired O2. Mb saturation was significantly lower in hypoxia than in normoxia [40 ± 3 vs. 49 ± 3% (SE)] throughout incremental exercise to maximal work rate. With the assumption of a PO2 at which 50% of Mb-binding sites are bound with O2 of 3.2 Torr, Mb-associated PO2 averaged 3.1 ± 0.3 and 2.3 ± 0.2 Torr in normoxia and hypoxia, respectively. Net blood lactate efflux was unrelated to intracellular PO2 across the range of incremental exercise to maximum (r = 0.03 and 0.07 in normoxia and hypoxia, respectively) but linearly related to O2 consumption (r = 0.97 and 0.99 in normoxia and hypoxia, respectively) with a greater slope in 12% O2. Net lactate efflux was also linearly related to intracellular pH (r = 0.94 and 0.98 in normoxia and hypoxia, respectively). These data suggest that with increasing work rate, at a given fraction of inspired O2, lactate efflux is unrelated to muscle cytoplasmic PO2, yet the efflux is higher in hypoxia. Catecholamine values from comparable studies are included and indicate that lactate efflux in hypoxia may be due to systemic rather than intracellular hypoxia.

blood flow; myoglobin; magnetic resonance spectroscopy; quadriceps; anaerobic threshold; knee-extensor exercise; diffusional conductance


This article has been cited by other articles:


Home page
 Am. J. Physiol. Cell Physiol.Home page
Y. Li, R. K. Dash, J. Kim, G. M. Saidel, and M. E. Cabrera
Role of NADH/NAD+ transport activity and glycogen store on skeletal muscle energy metabolism during exercise: in silico studies
Am J Physiol Cell Physiol, January 1, 2009; 296(1): C25 - C46.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
L. Nybo
Hyperthermia and fatigue
J Appl Physiol, March 1, 2008; 104(3): 871 - 878.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
L. J. Haseler, A. Lin, J. Hoff, and R. S. Richardson
Oxygen availability and PCr recovery rate in untrained human calf muscle: evidence of metabolic limitation in normoxia
Am J Physiol Regulatory Integrative Comp Physiol, November 1, 2007; 293(5): R2046 - R2051.
[Abstract] [Full Text] [PDF]

Home page
 Exp PhysiolHome page
R. A. Howlett and M. C. Hogan
Muscle: Effect of hypoxia on fatigue development in rat muscle composed of different fibre types
Exp Physiol, September 1, 2007; 92(5): 887 - 894.
[Abstract] [Full Text] [PDF]

Home page
 FASEB J.Home page
T. Hashimoto, R. Hussien, S. Oommen, K. Gohil, and G. A. Brooks
Lactate sensitive transcription factor network in L6 cells: activation of MCT1 and mitochondrial biogenesis
FASEB J, August 1, 2007; 21(10): 2602 - 2612.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
J. Barden, L. Lawrenson, J. G. Poole, J. Kim, D. W. Wray, D. M. Bailey, and R. S. Richardson
Limitations to vasodilatory capacity and VO2 max in trained human skeletal muscle
Am J Physiol Heart Circ Physiol, May 1, 2007; 292(5): H2491 - H2497.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
M. I. Lindinger, G. A. Brooks, G. C. Henderson, T. Hashimoto, T. Mau, J. A. Fattor, M. A. Horning, R. Hussien, H.-S. Cho, N. Faghihnia, et al.
Lactic acid accumulation is an advantage/disadvantage during muscle activity
J Appl Physiol, June 1, 2006; 100(6): 2100 - 2102.
[Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
J. Zoll, E. Ponsot, S. Dufour, S. Doutreleau, R. Ventura-Clapier, M. Vogt, H. Hoppeler, R. Richard, and M. Fluck
Exercise training in normobaric hypoxia in endurance runners. III. Muscular adjustments of selected gene transcripts
J Appl Physiol, April 1, 2006; 100(4): 1258 - 1266.
[Abstract] [Full Text] [PDF]

Home page
 J. Physiol.Home page
R. S. Richardson, S. Duteil, C. Wary, D. W. Wray, J. Hoff, and P. G. Carlier
Human skeletal muscle intracellular oxygenation: the impact of ambient oxygen availability
J. Physiol., March 1, 2006; 571(2): 415 - 424.
[Abstract] [Full Text] [PDF]

Home page
 J. Exp. Biol.Home page
A. Philp, A. L. Macdonald, and P. W. Watt
Lactate - a signal coordinating cell and systemic function
J. Exp. Biol., December 15, 2005; 208(24): 4561 - 4575.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
T. D. Noakes, J. A. L. Calbet, R. Boushel, H. Sondergaard, G. Radegran, P. D. Wagner, and B. Saltin
Central regulation of skeletal muscle recruitment explains the reduced maximal cardiac output during exercise in hypoxia
Am J Physiol Regulatory Integrative Comp Physiol, October 1, 2004; 287(4): R996 - R1002.
[Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
L. J. Haseler, A. P. Lin, and R. S. Richardson
Skeletal muscle oxidative metabolism in sedentary humans: 31P-MRS assessment of O2 supply and demand limitations
J Appl Physiol, September 1, 2004; 97(3): 1077 - 1081.
[Abstract] [Full Text] [PDF]

Home page
 J. Physiol.Home page
L. B. Gladden
Lactate metabolism: a new paradigm for the third millennium
J. Physiol., July 1, 2004; 558(1): 5 - 30.
[Abstract] [Full Text] [PDF]

Home page
 J. Physiol.Home page
S. A Jubrias, G. J Crowther, E. G Shankland, R. K Gronka, and K. E Conley
Acidosis inhibits oxidative phosphorylation in contracting human skeletal muscle in vivo
J. Physiol., December 1, 2003; 553(2): 589 - 599.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. GenomicsHome page
S. J. Prior, J. M. Hagberg, D. A. Phares, M. D. Brown, L. Fairfull, R. E. Ferrell, and S. M. Roth
Sequence variation in hypoxia-inducible factor 1{alpha} (HIF1A): association with maximal oxygen consumption
Physiol Genomics, September 29, 2003; 15(1): 20 - 26.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
J. A. L. Calbet, J. A. De Paz, N. Garatachea, S. Cabeza de Vaca, and J. Chavarren
Anaerobic energy provision does not limit Wingate exercise performance in endurance-trained cyclists
J Appl Physiol, February 1, 2003; 94(2): 668 - 676.
[Abstract] [Full Text] [PDF]

Home page
 J ANIM SCIHome page
J. M. Reecy, S. A. Miller, and M. Webster
Recent Advances That Impact Skeletal Muscle Growth and Development Research
J Anim Sci, January 1, 2003; 81(13_suppl_1): E1 - 8.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
R. S. Richardson, E. A. Noyszewski, B. Saltin, and J. Gonzalez-Alonso
Effect of mild carboxy-hemoglobin on exercising skeletal muscle: intravascular and intracellular evidence
Am J Physiol Regulatory Integrative Comp Physiol, November 1, 2002; 283(5): R1131 - R1139.
[Abstract] [Full Text] [PDF]

Home page
 J. Physiol.Home page
P J LeBlanc, M L Parolin, N L Jones, and G J F Heigenhauser
Effects of respiratory alkalosis on human skeletal muscle metabolism at the onset of submaximal exercise
J. Physiol., October 1, 2002; 544(1): 303 - 313.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
R. S. Richardson, S. C. Newcomer, and E. A. Noyszewski
Skeletal muscle intracellular PO2 assessed by myoglobin desaturation: response to graded exercise
J Appl Physiol, December 1, 2001; 91(6): 2679 - 2685.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
S. Perrey, M. E. Tschakovsky, and R. L. Hughson
Muscle chemoreflex elevates muscle blood flow and O2 uptake at exercise onset in nonischemic human forearm
J Appl Physiol, November 1, 2001; 91(5): 2010 - 2016.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
R. A. Howlett and M. C. Hogan
Intracellular PO2 decreases with increasing stimulation frequency in contracting single Xenopus muscle fibers
J Appl Physiol, August 1, 2001; 91(2): 632 - 636.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
L. B. Gladden
Lactic acid: New roles in a new millennium
PNAS, January 16, 2001; 98(2): 395 - 397.
[Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
W. F. Kemper, S. L. Lindstedt, L. K. Hartzler, J. W. Hicks, and K. E. Conley
Shaking up glycolysis: Sustained, high lactate flux during aerobic rattling
PNAS, December 14, 2000; (2000) 11387598.
[Abstract] [Full Text]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
R. S. Richardson
Complementary studies of exercised-induced angiogenic growth factors in human skeletal muscle
Am J Physiol Heart Circ Physiol, December 1, 2000; 279 (6): H3144 - H3145.
[Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
M. J. Brosnan, D. T. Martin, A. G. Hahn, C. J. Gore, and J. A. Hawley
Impaired interval exercise responses in elite female cyclists at moderate simulated altitude
J Appl Physiol, November 1, 2000; 89(5): 1819 - 1824.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
M. L. Parolin, L. L. Spriet, E. Hultman, M. P. Matsos, M. G. Hollidge-Horvat, N. L. Jones, and G. J. F. Heigenhauser
Effects of PDH activation by dichloroacetate in human skeletal muscle during exercise in hypoxia
Am J Physiol Endocrinol Metab, October 1, 2000; 279(4): E752 - E761.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
D. D. Fuller and R. F. Fregosi
Fatiguing contractions of tongue protrudor and retractor muscles: influence of systemic hypoxia
J Appl Physiol, June 1, 2000; 88(6): 2123 - 2130.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
R. S. Richardson; and T. Jue
Intracellular PO2 and bioenergetic measurements in skeletal muscle: the role of exercise paradigm
Am J Physiol Regulatory Integrative Comp Physiol, April 1, 2000; 278(4): R1111 - R1113.
[Full Text] [PDF]

Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
M. L. Parolin, L. L. Spriet, E. Hultman, M. G. Hollidge-Horvat, N. L. Jones, and G. J. F. Heigenhauser
Regulation of glycogen phosphorylase and PDH during exercise in human skeletal muscle during hypoxia
Am J Physiol Endocrinol Metab, March 1, 2000; 278(3): E522 - E534.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
R. S. Richardson, J. S. Leigh, P. D. Wagner, and E. A. Noyszewski
Cellular PO2 as a determinant of maximal mitochondrial O2 consumption in trained human skeletal muscle
J Appl Physiol, July 1, 1999; 87(1): 325 - 331.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
B. Grassi, V. Quaresima, C. Marconi, M. Ferrari, and P. Cerretelli
Blood lactate accumulation and muscle deoxygenation during incremental exercise
J Appl Physiol, July 1, 1999; 87(1): 348 - 355.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
L. J. Haseler, M. C. Hogan, and R. S. Richardson
Skeletal muscle phosphocreatine recovery in exercise-trained humans is dependent on O2 availability
J Appl Physiol, June 1, 1999; 86(6): 2013 - 2018.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
R. S. Richardson; and F. Maltais
Skeletal Muscle Dysfunction vs. Muscle Disuse in Patients With COPD
J Appl Physiol, May 1, 1999; 86(5): 1751 - 1752.
[Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
W. F. Kemper, S. L. Lindstedt, L. K. Hartzler, J. W. Hicks, and K. E. Conley
From the Cover: Shaking up glycolysis: Sustained, high lactate flux during aerobic rattling
PNAS, January 16, 2001; 98(2): 723 - 728.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online