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J Appl Physiol 85: 47-52, 1998;
8750-7587/98 $5.00
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Vol. 85, Issue 1, 47-52, July 1998

Functional diameters of alveolar microvessels at high lung volume in zone II

Robert L. Conhaim and Lance A. Rodenkirch

Department of Surgery, University of Wisconsin-Madison, Madison, Wisconsin 53705

To estimate the functional diameter of alveolar microvessels, we perfused isolated rat lungs with fluorescent latex particles (1 diameter/lung) at inflation, pulmonary arterial, and left atrial pressures of 25, 30, and 0 cmH2O, respectively. We used confocal microscopy to count latex particles within septal microvessels and flow cytometry to count particle concentrations in venous outflow. We found 1-, 2-, and 4-µm-diameter particles within septal vessels of 45 ± 12, 31 ± 12, and 25 ± 9%, respectively, of examined alveoli. Particles of 5-µm diameter were absent from septal vessels but were present within a small percentage of corner vessels. Particle concentrations in the venous outflow for 1-, 2-, 4-, and 5-µm-diameter particles were 54 ± 28, 67 ± 32, 2.2 ± 0.3, and 0.4 ± 0.3%, respectively, of the arterial inflow. Particles with diameters of 6 or 10 µm were absent from venous outflow. Our results suggest that, under these conditions, the functional diameter of the septal microvessels is ~4 µm and that the diameter of the adjacent corner vessels is slightly larger but <6 µm.

isolated rat lungs; confocal microscopy; rapid freezing; pulmonary microcirculation; latex particle perfusion


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