Journal of Applied Physiology Ad Instruments
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

J Appl Physiol 85: 372-376, 1998;
8750-7587/98 $5.00
This Article
Right arrow Full Text Free
Right arrow Full Text (PDF) Free
Right arrow Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Gozal, E.
Right arrow Articles by Gozal, D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Gozal, E.
Right arrow Articles by Gozal, D.
Vol. 85, Issue 1, 372-376, July 1998

RAPID COMMUNICATION
NF-kappa B induction during in vivo hypoxia in dorsocaudal brain stem of rat: effect of MK-801 and L-NAME

Evelyne Gozal, Narong Simakajornboon, and David Gozal

Constance S. Kaufman Pediatric Pulmonary Research Laboratory, Departments of Pediatrics and Physiology, Tulane University School of Medicine, New Orleans, Louisiana 70112

In the nucleus of the solitary tract, NMDA receptors are critical for the hypoxic ventilatory response while neuronal nitric oxide synthase (NOS) modulates the late component of this response. Nuclear factor (NF)-kappa B is a ubiquitous transcription factor that increases the expression of multiple stress-activated genes. We sought to examine temporal changes in expression of NF-kappa B within the dorsocaudal brain stem of conscious rats after exposures to 10% O2. Time-dependent increases in NF-kappa B occurred with hypoxia and peaked at 60 min. Pretreatment with the N-methyl-D-aspartate (NMDA)-receptor channel antagonist dizocilpine maleate (MK-801) markedly attenuated NF-kappa B complexes during hypoxia. In contrast, after NOS inhibition with NG-nitro-L-arginine methyl ester (L-NAME), although NF-kappa B was diminished in normoxia, increased NF-kappa B expression still occurred with hypoxia. Increased phosphorylation of the NF-kappa B regulatory unit [inhibitory (I)kappa B] was detected by immunoblotting and also peaked at 60 min. Phosphorylation of Ikappa -B during hypoxia was attenuated by MK-801 but not by L-NAME. Thus NMDA-receptor activation in the dorsocaudal brain stem during hypoxia elicits in NF-kappa B activity marked enhancements that are unaffected after NOS blockade.

nuclear factor-kappa B; NG-nitro-L-arginine methyl ester; hypoxia; transcription factors; nitric oxide; glutamate; N-methyl-D-aspartate


This article has been cited by other articles:


Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
P. M. de Paula, G. Tolstykh, and S. Mifflin
Chronic intermittent hypoxia alters NMDA and AMPA-evoked currents in NTS neurons receiving carotid body chemoreceptor inputs
Am J Physiol Regulatory Integrative Comp Physiol, June 1, 2007; 292(6): R2259 - R2265.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
L. del Peso, M. C. Castellanos, E. Temes, S. Martin-Puig, Y. Cuevas, G. Olmos, and M. O. Landazuri
The von Hippel Lindau/Hypoxia-inducible Factor (HIF) Pathway Regulates the Transcription of the HIF-Proline Hydroxylase Genes in Response to Low Oxygen
J. Biol. Chem., December 5, 2003; 278(49): 48690 - 48695.
[Abstract] [Full Text] [PDF]

Home page
 J. Am. Soc. Nephrol.Home page
G. K. RANGAN, Y. WANG, and D. C. H. HARRIS
Pharmacologic Modulators of Nitric Oxide Exacerbate Tubulointerstitial Inflammation in Proteinuric Rats
J. Am. Soc. Nephrol., August 1, 2001; 12(8): 1696 - 1705.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
N. S. Chandel, W. C. Trzyna, D. S. McClintock, and P. T. Schumacker
Role of Oxidants in NF-{kappa}B Activation and TNF-{alpha} Gene Transcription Induced by Hypoxia and Endotoxin
J. Immunol., July 15, 2000; 165(2): 1013 - 1021.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S.-F. Yan, J. Lu, Y. S. Zou, J. Soh-Won, D. M. Cohen, P. M. Buttrick, D. R. Cooper, S. F. Steinberg, N. Mackman, D. J. Pinsky, et al.
Hypoxia-associated Induction of Early Growth Response-1 Gene Expression
J. Biol. Chem., May 21, 1999; 274(21): 15030 - 15040.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
D. Gozal and E. Gozal
Episodic hypoxia enhances late hypoxic ventilation in developing rat: putative role of neuronal NO synthase
Am J Physiol Regulatory Integrative Comp Physiol, January 1, 1999; 276(1): R17 - R22.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online