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1 Department of Human Biology and Nutritional Sciences, University of Guelph, Guelph, Ontario N1G 2W1; and 2 Department of Medicine, McMaster University, Hamilton, Ontario, Canada L8N 3Z5
This study was designed to compare the
activity of skeletal muscle carnitine palmitoyltransferase I (CPT I) in
trained and inactive men (n = 14) and
women (n = 12). CPT I
activity was measured in intact mitochondria, isolated from needle
biopsy vastus lateralis muscle samples (~60 mg). The variability of
CPT I activity determined on two biopsy samples from the same leg on
the same day was 4.4, whereas it was 7.0% on two biopsy samples from
the same leg on different days. The method was sensitive to the CPT I
inhibitor malonyl-CoA (88% inhibition) and therefore specific for CPT
I activity. The mean CPT I activity for all 26 subjects was 141.1 ± 10.6 µmol · min
1 · kg
wet muscle (wm)1 and was
not different when all men vs. all women (140.5 ± 15.7 and 142.2 ± 14.5 µmol · min1 · kg
wm1, respectively) were compared. However, CPT I
activity was significantly higher in trained vs. inactive subjects for
both men (176.2 ± 21.1 vs. 104.1 ± 13.6 µmol · min1 · kg
wm1) and women (167.6 ± 14.1 vs. 91.2 ± 9.5 µmol · min1 · kg
wm1). CPT I activity was also significantly correlated
with citrate synthase activity (all subjects,
r = 0.76) and maximal oxygen consumption expressed in milliliters per kilogram per minute (all subjects, r = 0.69). The
results of this study suggest that CPT I activity can be accurately and
reliably measured in intact mitochondria isolated from human muscle
biopsy samples. CPT I activity was not affected by gender, and higher
activities in aerobically trained subjects appeared to be the result of
increased mitochondrial content in both men and women.
long-chain fatty acid transport; vastus lateralis muscle; aerobic training; malonyl-CoA; citrate synthase activity
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