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J Appl Physiol 85: 139-147, 1998;
8750-7587/98 $5.00
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Vol. 85, Issue 1, 139-147, July 1998

Spaceflight modulates insulin-like growth factor binding proteins and glucocorticoid receptor in osteoblasts

Yasuhiro Kumei1, Hitoyata Shimokawa1, Hisako Katano1, Hideo Akiyama2, Masahiko Hirano2, Chiaki Mukai3, Shunji Nagaoka3, Peggy A. Whitson4, and Clarence F. Sams4

1 Faculty of Dentistry, Tokyo Medical and Dental University, Tokyo 113-8549; 2 Toray Research Center, Kamakura 248-78555; 3 National Space Development Agency, Tsukuba Space Center, Tsukuba 305-0047, Japan; and 4 National Aeronautics and Space Administration, Johnson Space Center, Houston, Texas 77058

Rat osteoblasts were cultured for 4 or 5 days during a Space Shuttle mission. After 20-h treatment with 1alpha ,25-dihydroxyvitamin D3, conditioned media were harvested and cellular DNA and/or RNA were fixed on board. The insulin-like growth factor binding protein (IGF BP)-3 levels in the media were three- and tenfold higher than in ground controls on the fourth and fifth flight days, as quantitated by Western ligand blotting and radioimmunoassay, respectively. The increased IGF BP-3 protein levels correlated with two- to threefold elevation of IGF BP-3 mRNA levels, obtained by reverse transcription-polymerase chain reaction. The IGF BP-5 mRNA levels in flight cultures were 33-69% lower than in ground controls. The IGF BP-4 mRNA levels in flight cultures were 75% lower than in ground controls on the fifth day but were not different on the fourth day. The glucocorticoid receptor mRNA levels in flight cultures were increased by three- to eightfold on the fourth and fifth days compared with levels in ground controls. These data suggest potential mechanisms underlying spaceflight-induced osteopenia.

bone demineralization; osteoblast; microgravity


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