Journal of Applied Physiology AJP: Renal Physiology
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J Appl Physiol 84: 1788-1794, 1998;
8750-7587/98 $5.00
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Vol. 84, Issue 5, 1788-1794, May 1998

Contraction-induced increase in Vmax of palmitate uptake and oxidation in perfused skeletal muscle

Lorraine P. Turcotte1, Carsten Petry2, Bente Kiens2, and Erik A. Richter2

1 Department of Exercise Sciences, University of Southern California, Los Angeles, California 90089-0652; and 2 The Copenhagen Muscle Research Centre, August Krogh Institute, University of Copenhagen, DK-2100 Copenhagen, Denmark

To evaluate the effects of contractions on the kinetics of uptake and oxidation of palmitate in a physiological muscle preparation, rat hindquarters were perfused with glucose (6 mmol/l), albumin-bound [1-14C]palmitate, and varying amounts of albumin-bound palmitate (200-2,200 µmol/l) at rest and during muscle contractions. When plotted against the unbound palmitate concentration, palmitate uptake and oxidation displayed simple Michaelis-Menten kinetics with estimated maximal velocity (Vmax) and Michaelis-Menten constant (Km) values of 42.8 ± 3.8 (SE) nmol · min-1 · g-1 and 13.4 ± 3.4 nmol/l for palmitate uptake and 3.8 ± 0.4 nmol · min-1 · g-1 and 8.1 ± 2.9 nmol/l for palmitate oxidation, respectively, at rest. Whereas muscle contractions increased the Vmax for both palmitate uptake and oxidation to 91.6 ± 10.1 and 16.5 ± 2.3 nmol · min-1 · g-1, respectively, the Km remained unchanged. Vmax and Km estimates obtained from Hanes-Woolf plots (substrate concentration/velocity vs. substrate concentration) were not significantly different. In the resting perfused hindquarter, an increase in palmitate delivery from 31.9 ± 0.9 to 48.7 ± 1.2 µmol · g-1 · h-1 by increasing perfusate flow was associated with a decrease in the fractional uptake of palmitate so that the rates of uptake and oxidation of palmitate remained unchanged. It is concluded that the rates of uptake and oxidation of long-chain fatty acids (LCFA) saturate with an increase in the concentration of unbound LCFA in perfused skeletal muscle and that muscle contractions, but not an increase in plasma flow, increase the Vmax for LCFA uptake and oxidation. The data are consistent with the notion that uptake of LCFA in muscle may be mediated in part by a transport system.

electrical stimulation; fatty acid metabolism; fatty acid transport; fatty acid uptake; hindquarter perfusion; skeletal muscle


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