| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Vol. 84, Issue 3, 908-913, March 1998
Human Biology and Nutritional Sciences, University of Guelph, Guelph, Ontario, Canada N1G 2W1
Traditionally, there have been two methods for measuring total muscle glycogen (Glytot), either by acid hydrolysis (AC) or by enzymatic hydrolysis (EZ). As well, it has been determined that rodent muscle contains two pools of glycogen, macroglycogen (MG) and proglycogen (PG). This MG/PG determination of Glytot has never been compared with AC or EZ methods, nor has it been determined whether the two pools exist in human skeletal muscle. A detailed comparison of the three methods was performed by using both rodent and human muscle. It was found that repeated analysis of independent portions of muscle generally gave coefficients of variation of <10%. The PG fraction was always in excess of MG, which was 6-10% of Glytot in rodent muscle and in human samples when Glytot was low but increased to ~40% when Glytot was high. It was found that AC and EZ Glytot were not statistically different (P < 0.05), nor was there a difference between the MG+PG Glytot and that determined by AC or EZ. The Glytot from MG+PG extraction had a strong correlation with the values obtained by either AC (r = 1.0) or EZ (r = 0.96). These data suggest that MG+PG do exist in human skeletal muscle and can be measured reliably in biopsy-sized samples. All three methods give an accurate representation of human Glytot and are comparable in their precision.
metabolic pools; glycogenin; measurement techniques; carbohydrate; biopsy
This article has been cited by other articles:
|
|
 |
|
  P. C. Kienesberger, D. Lee, T. Pulinilkunnil, D. S. Brenner, L. Cai, C. Magnes, H. C. Koefeler, I. E. Streith, G. N. Rechberger, G. Haemmerle, et al. Adipose Triglyceride Lipase Deficiency Causes Tissue-specific Changes in Insulin Signaling J. Biol. Chem., October 30, 2009; 284(44): 30218 - 30229. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. S. Mathai, A. Bonen, C. R. Benton, D. L. Robinson, and T. E. Graham Rapid exercise-induced changes in PGC-1{alpha} mRNA and protein in human skeletal muscle J Appl Physiol, October 1, 2008; 105(4): 1098 - 1105. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. J. Wilson, J. E. Gusba, D. L. Robinson, and T. E. Graham Glycogenin protein and mRNA expression in response to changing glycogen concentration in exercise and recovery Am J Physiol Endocrinol Metab, June 1, 2007; 292(6): E1815 - E1822. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 I. Marchand, M. Tarnopolsky, K. B. Adamo, J. M. Bourgeois, K. Chorneyko, and T. E. Graham Quantitative assessment of human muscle glycogen granules size and number in subcellular locations during recovery from prolonged exercise J. Physiol., April 15, 2007; 580(2): 617 - 628. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. C. Devries, M. J. Hamadeh, S. M. Phillips, and M. A. Tarnopolsky Menstrual cycle phase and sex influence muscle glycogen utilization and glucose turnover during moderate-intensity endurance exercise Am J Physiol Regulatory Integrative Comp Physiol, October 1, 2006; 291(4): R1120 - R1128. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. I Martinelli, N. O Mocchiutti, and C. A Bernal Dietary di(2-ethylhexyl)phthalate-impaired glucose metabolism in experimental animals Human and Experimental Toxicology, September 1, 2006; 25(9): 531 - 538. [Abstract] [PDF]
|
|
|
|
 |
|
 G. Bee, C. Biolley, G. Guex, W. Herzog, S. M. Lonergan, and E. Huff-Lonergan Effects of available dietary carbohydrate and preslaughter treatment on glycolytic potential, protein degradation, and quality traits of pig muscles J Anim Sci, January 1, 2006; 84(1): 191 - 203. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. C. Devries, M. J. Hamadeh, T. E. Graham, and M. A. Tarnopolsky 17{beta}-Estradiol Supplementation Decreases Glucose Rate of Appearance and Disappearance with No Effect on Glycogen Utilization during Moderate Intensity Exercise in Men J. Clin. Endocrinol. Metab., November 1, 2005; 90(11): 6218 - 6225. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Shearer, T. E. Graham, D. S. Battram, D. L. Robinson, E. A. Richter, R. J. Wilson, and M. Bakovic Glycogenin activity and mRNA expression in response to volitional exhaustion in human skeletal muscle J Appl Physiol, September 1, 2005; 99(3): 957 - 962. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Shearer, R. J. Wilson, D. S. Battram, E. A. Richter, D. L. Robinson, M. Bakovic, and T. E. Graham Increases in glycogenin and glycogenin mRNA accompany glycogen resynthesis in human skeletal muscle Am J Physiol Endocrinol Metab, September 1, 2005; 289(3): E508 - E514. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S.-L. Wee, C. Williams, K. Tsintzas, and L. Boobis Ingestion of a high-glycemic index meal increases muscle glycogen storage at rest but augments its utilization during subsequent exercise J Appl Physiol, August 1, 2005; 99(2): 707 - 714. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. T. Waylan, J. D. Dunn, B. J. Johnson, J. P. Kayser, and E. K. Sissom Effect of flax supplementation and growth promotants on lipoprotein lipase and glycogenin messenger RNA concentrations in finishing cattle J Anim Sci, June 1, 2004; 82(6): 1868 - 1875. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. S. Battram, J. Shearer, D. Robinson, and T. E. Graham Caffeine ingestion does not impede the resynthesis of proglycogen and macroglycogen after prolonged exercise and carbohydrate supplementation in humans J Appl Physiol, March 1, 2004; 96(3): 943 - 950. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Rosenvold, B. Essen-Gustavsson, and H. J. Andersen Dietary manipulation of pro- and macroglycogen in porcine skeletal muscle J Anim Sci, January 1, 2003; 81(1): 130 - 134. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. S.L. Thong, W. Derave, B. Kiens, T. E. Graham, B. Urso, J. F.P. Wojtaszewski, B. F. Hansen, and E. A. Richter Caffeine-Induced Impairment of Insulin Action but Not Insulin Signaling in Human Skeletal Muscle Is Reduced by Exercise Diabetes, March 1, 2002; 51(3): 583 - 590. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. A. Tarnopolsky, C. Zawada, L. B. Richmond, S. Carter, J. Shearer, T. Graham, and S. M. Phillips Gender differences in carbohydrate loading are related to energy intake J Appl Physiol, July 1, 2001; 91(1): 225 - 230. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. E. Graham, K. B. Adamo, J. Shearer, I. Marchand, and B. Saltin Pro- and macroglycogenolysis: relationship with exercise intensity and duration J Appl Physiol, March 1, 2001; 90(3): 873 - 879. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Shearer, I. Marchand, M. A. Tarnopolsky, D. J. Dyck, and T. E. Graham Pro- and macroglycogenolysis during repeated exercise: roles of glycogen content and phosphorylase activation J Appl Physiol, March 1, 2001; 90(3): 880 - 888. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Greer, D. Friars, and T. E. Graham Comparison of caffeine and theophylline ingestion: exercise metabolism and endurance J Appl Physiol, November 1, 2000; 89(5): 1837 - 1844. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Kristiansen, J. Gade, J. F. P. Wojtaszewski, B. Kiens, and E. A. Richter Glucose uptake is increased in trained vs. untrained muscle during heavy exercise J Appl Physiol, September 1, 2000; 89(3): 1151 - 1158. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. L. Bowtell, K. Gelly, M. L. Jackman, A. Patel, M. Simeoni, and M. J. Rennie Effect of different carbohydrate drinks on whole body carbohydrate storage after exhaustive exercise J Appl Physiol, May 1, 2000; 88(5): 1529 - 1536. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. F. Hansen, W. Derave, P. Jensen, and E. A. Richter No limiting role for glycogenin in determining maximal attainable glycogen levels in rat skeletal muscle Am J Physiol Endocrinol Metab, March 1, 2000; 278(3): E398 - E404. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Shearer, I. Marchand, P. Sathasivam, M. A. Tarnopolsky, and T. E. Graham Glycogenin activity in human skeletal muscle is proportional to muscle glycogen concentration Am J Physiol Endocrinol Metab, January 1, 2000; 278(1): E177 - E180. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Asp, J. R. Daugaard, T. Rohde, K. Adamo, and T. Graham Muscle glycogen accumulation after a marathon: roles of fiber type and pro- and macroglycogen J Appl Physiol, February 1, 1999; 86(2): 474 - 478. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. B. Adamo, M. A. Tarnopolsky, and T. E. Graham Dietary carbohydrate and postexercise synthesis of proglycogen and macroglycogen in human skeletal muscle Am J Physiol Endocrinol Metab, August 1, 1998; 275(2): E229 - E234. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Visit Other APS Journals Online |
