Journal of Applied Physiology Fuel your research with LabChart
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

J Appl Physiol 84: 740-745, 1998;
8750-7587/98 $5.00
This Article
Right arrow Full Text Free
Right arrow Full Text (PDF) Free
Right arrow Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Lasnier, J. M.
Right arrow Articles by Wangensteen, O. D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Lasnier, J. M.
Right arrow Articles by Wangensteen, O. D.

Vol. 84, Issue 2, 740-745, February 1998

SPECIAL COMMUNICATION
Perfusion technique determines alveolar fluid resorption rate in the isolated perfused rat lung

Joseph M. Lasnier, David H. Ingbar, Ethan P. Carter, Kirk Wilson, Scott McKnite, Keith G. Lurie and O. Douglas Wangensteen

Departments of Medicine and Physiology, School of Medicine, University of Minnesota, Minneapolis, Minnesota 55455

Lasnier, Joseph M., David H. Ingbar, Ethan P. Carter, Kirk Wilson, Scott McKnite, Keith G. Lurie, and O. Douglas Wangensteen. Perfusion technique determines alveolar fluid resorption rate in the isolated perfused rat lung. J. Appl. Physiol. 84(2): 740-745, 1998.---The isolated perfused lung (IPL) preparation is a well-established model for the study of alveolar epithelial sodium transport. We noted that preparations of normal fluid-filled rat lungs with recirculated perfusate reproducibly lost weight, whereas preparations in which the perfusate was discarded after a single pass through the lungs had a variable and lesser weight change. To confirm this, we performed IPL experiments by using male Sprague-Dawley specific-pathogen-free rats (175-225 g). In 10 IPLs, perfusate initially was discarded after passing through the lungs and then was recirculated continuously. During the single-pass period, the rate of weight change was +0.7 ± 2.0 mg/min compared with -9.0 ± 1.3 mg/min for the recirculating period. Adenosine 3',5'-cyclic monophosphate (cAMP) accumulated during recirculation. The weight loss induced by recirculation was reproduced by perfusion with 8-bromoadenosine 3',5'-cyclic monophosphate or terbutaline in single-pass fashion and blocked when the kinase inhibitor H-8 or phosphodiesterase was present in the recirculating perfusate. In summary, perfusate recirculation in the IPL stimulates fluid resorption at least partially via cAMP. This should be factored into the design and interpretation of IPL experiments.

isolated perfused lung; recirculation; adenosine 3',5'-cyclic monophosphate; active sodium transport; pulmonary edema

The Journal of Applied Physiology 84(2):740-745
8750-7587/98 $5.00 Copyright © 1998 the American Physiological Society


This article has been cited by other articles:


Home page
 J. Biol. Chem.Home page
J. Lei, C. N. Mariash, and D. H. Ingbar
3,3',5-Triiodo-L-thyronine Up-regulation of Na,K-ATPase Activity and Cell Surface Expression in Alveolar Epithelial Cells Is Src Kinase- and Phosphoinositide 3-Kinase-dependent
J. Biol. Chem., November 12, 2004; 279(46): 47589 - 47600.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
J. I. Sznajder, P. Factor, and D. H. Ingbar
Lung Edema Clearance: 20 Years of Progress: Invited Review: Lung edema clearance: role of Na+-K+-ATPase
J Appl Physiol, November 1, 2002; 93(5): 1860 - 1866.
[Abstract] [Full Text] [PDF]

Home page
 Eur Respir JHome page
M. McGuire and A. Bradford
Chronic intermittent hypercapnic hypoxia increases pulmonary arterial pressure and haematocrit in rats
Eur. Respir. J., August 1, 2001; 18(2): 279 - 285.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
M. L. Barnard; and J. M. Lasnier
Perfusion Techniques for Determining Alveolar Fluid Resorption Rate
J Appl Physiol, May 1, 1999; 86(5): 1749 - 1750.
[Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
F. J. Saldias, A. Comellas, C. Guerrero, K. M. Ridge, D. H. Rutschman, and J. I. Sznajder
Time course of active and passive liquid and solute movement in the isolated perfused rat lung model
J Appl Physiol, October 1, 1998; 85(4): 1572 - 1577.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online