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Vol. 83, Issue 6, 2064-2072, December 1997
Department of Human Biology, Maastricht University, 6200 MD Maastricht, The Netherlands
Received 12 March 1997; accepted in final form 23 July 1997.
Schoffelen, Paul F. M., Klaas R. Westerterp, Wim H. M. Saris, and Foppe Ten Hoor. A dual-respiration chamber
system with automated calibration. J. Appl.
Physiol. 83(6): 2064-2072, 1997.
This study
characterizes respiration chambers with fully automated calibration.
The system consists of two 14-m3
pull-type chambers. Care was taken to provide a friendly environment for the subjects, with the possibility of social contact during the
experiment. Gas analysis was automated to correct for analyzer drift
and barometric pressure variations and to provide ease of use. Methods
used for checking the system's performance are described. The
gas-analysis repeatability was within 0.002%. Results of alcohol combustion (50-350 ml/min
CO2) show an accuracy of 0.5 ± 2.0 (SD) % for O2
consumption and 
0.3 ± 1.6% for
CO2 production for 2- to 24-h
experiments. It is concluded that response time is not the main factor
with respect to the smallest practical measurement interval (duration);
volume, mixing, gas-analysis accuracy, and levels of
O2 consumption and
CO2 production are at least
equally important. The smallest practical interval was 15-25 min,
as also found with most chamber systems described in the literature. We chose to standardize 0.5 h as the minimum measurement
interval.
indirect calorimetry; energy expenditure; oxygen consumption and carbon dioxide production; measurement interval
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