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Vol. 83, Issue 6, 2043-2047, December 1997
Division of Health Promotion, National Institute of Health and Nutrition, Toyama 1-23-1, Shinjuku-City, Tokyo 162; and Institute of Health and Sport Sciences, University of Tsukuba, Tennoudai 1-1-1, Tsukuba-City, Ibaraki 305, Japan
Received 7 October 1996; accepted in final form 25 July 1997.
Kawanaka, Kentaro, Izumi Tabata, Shigeru Katsuta, and
Mitsuru Higuchi. Changes in insulin-stimulated glucose transport and GLUT-4 protein in rat skeletal muscle after training.
J. Appl. Physiol. 83(6):
2043-2047, 1997.
After running training, which increased GLUT-4
protein content in rat skeletal muscle by <40% compared with control
rats, the training effect on insulin-stimulated maximal glucose
transport (insulin responsiveness) in skeletal muscle was short lived
(24 h). A recent study reported that GLUT-4 protein content in rat
epitrochlearis muscle increased dramatically (~2-fold) after swimming
training (J.-M. Ren, C. F. Semenkovich, E. A. Gulve, J. Gao, and
J. O. Holloszy. J. Biol.
Chem. 269, 14396-14401, 1994).
Because GLUT-4 protein content is known to be closely related to
skeletal muscle insulin responsiveness, we thought it possible that the
training effect on insulin responsiveness may remain for >24 h after
swimming training if GLUT-4 protein content decreases gradually from
the relatively high level and still remains higher than control level
for >24 h after swimming training. Therefore, we examined this
possibility. Male Sprague-Dawley rats swam 2 h a day for 5 days with a
weight equal to 2% of body mass. Approximately 18, 42, and 90 h after
cessation of training, GLUT-4 protein concentration and
2-[1,2-3H]deoxy-D-glucose
transport in the presence of a maximally stimulating concentration of
insulin (2 mU/ml) were examined by using incubated epitrochlearis
muscle preparation. Swimming training increased GLUT-4 protein
concentration and insulin responsiveness by 87 and 85%, respectively,
relative to age-matched controls when examined 18 h after training.
Forty-two hours after training, GLUT-4 protein concentration and
insulin responsiveness were still higher by 52 and 51%, respectively,
in muscle from trained rats compared with control. GLUT-4 protein
concentration and insulin responsiveness in trained muscle returned to
sedentary control level within 90 h after training. We conclude that
1) the change in insulin
responsiveness during detraining is directly related to muscle GLUT-4
protein content, and 2)
consequently, the greater the increase in GLUT-4 protein content that
is induced by training, the longer an effect on insulin responsiveness
persists after the training.
insulin responsiveness; epitrochlearis; isolated muscle incubation; detraining
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