Journal of Applied Physiology
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J Appl Physiol 83: 1842-1848, 1997;
8750-7587/97 $5.00
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Vol. 83, Issue 6, 1842-1848, December 1997

Redox behavior of cytochrome oxidase in the rat brain measured by near-infrared spectroscopy

Yoko Hoshi, Osamu Hazeki, Yasuyuki Kakihana, and Mamoru Tamura

Biophysics Group, Research Institute for Electronic Science, Hokkaido University, Sapporo 060, Japan

Received 9 December 1996; accepted in final form 21 July 1997.

Hoshi, Yoko, Osamu Hazeki, Yasuyuki Kakihana, and Mamoru Tamura. Redox behavior of cytochrome oxidase in the rat brain measured by near-infrared spectroscopy. J. Appl. Physiol. 83(6): 1842-1848, 1997.---Using near-infrared spectroscopy, we developed a new approach for measuring the redox state of cytochrome oxidase in the brain under normal blood-circulation conditions. Our algorithm does not require the absorption coefficient of cytochrome oxidase, which differs from study to study. We employed this method for evaluation of effects of changes in oxygen delivery on cerebral oxygenation in rats. When fractional inspired oxygen was decreased in a stepwise manner from 100 to <10%, at which point the concentration of oxygenated hemoglobin ([HbO2]) decreased by ~60%, cytochrome oxidase started to be reduced. Increases in arterial PO2 under hyperoxic conditions caused an increase in [HbO2], whereas further oxidation of cytochrome oxidase was not observed. The dissociation of the responses of hemogloblin and cytochrome oxidase was also clearly observed after the injection of epinephrine under severely hypoxic conditions; that is, cytochrome oxidase was reoxidized with increasing blood pressure, whereas hemoglobin oxygenation was not changed. These data indicated that oxygen-dependent redox changes in cytochrome oxidase occur only when oxygen delivery is extremely impaired. This is consistent with the in vitro data of our previous study.

mitochondria; oxygen delivery; hypoxia; hemoglobin oxygenation

0161-7567/97 $5.00 Copyright © 1997 the American Physiological Society




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