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Vol. 83, Issue 5, 1697-1705, 1997
1 Department of Physiology, University of South Alabama, Mobile, Alabama 36688; and 2 Department of Medicine, University of California, San Diego, La Jolla, California 92093
Received 16 May 1997; accepted in final form 17 July 1997.
Parker, James C., Ellen C. Breen, and John B. West.
High vascular and airway pressures increase interstitial protein mRNA expression in isolated rat lungs. J. Appl.
Physiol. 83(5): 1697-1705, 1997.
We hypothesized
that wall stresses produced by high peak airway (Paw) and venous (Ppv)
pressures would increase mRNA levels for structural proteins of the
interstitial matrix in isolated rat lungs. Groups of lungs
(n = 6) were perfused for 4 h at a
peak Paw of 35 cmH2O (HiPaw),
cyclical peak Ppv of 28 cmH2O
(HiPv), or baseline vascular and airway pressures (LoPress). In two
separate groups, comparable peak pressures increased capillary filtration coefficient fourfold in each group. Northern blots were
probed for mRNA of 
1(I),
1(III), and
2(IV) procollagen chains,
laminin B chain, fibronectin, and transforming growth factor-
1, and densities were
normalized to 18S rRNA. mRNA was significantly higher in the HiPv group
for type I (4.3-fold) and type III (3.8-fold) procollagen and laminin B
chain (4.8-fold) and in the HiPaw group for type I (2.4-fold) and type
IV (4.5-fold) procollagen and laminin B chain (2.3-fold) than in the
LoPress group. Only fibronectin mRNA was significantly increased
(3.9-fold) in the LoPress group relative to unperfused lungs. Estimated
wall stresses were highest for alveolar septa in the HiPaw group and for capillaries in the HiPv group. The different patterns of mRNA expression are attributed to different regional stresses or extent of
injury.
collagen; fibrosis; pulmonary hypertension; barotrauma; fibronectin; vascular permeability
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