| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Asthma, Allergy, and Immunological Disease Research Center, Section of Pulmonary and Critical Care Medicine, Department of Medicine, and the Committees on Clinical Pharmacology, Cell Physiology, and Comparative Medicine and Pathology, Division of the Biological Sciences, The University of Chicago, Chicago, Illinois 60637; and Zentrum für Pneumologie und Thoraxchirurgie, Krankenhaus Grosshansdorf, LVA Hamburg, D-22927 Grosshansdorf, Germany
Received 15 April 1997; accepted in final form 17 June 1997.
Mitchell, R. W., K. F. Rabe, H. Magnussen, and A. R. Leff.
Passive sensitization of human airways induces myogenic contractile responses in vitro. J. Appl.
Physiol. 83(4): 1276-1281, 1997.
We assessed
effects of passive sensitization on human bronchial smooth muscle (BSM)
response to mechanical stretching in vitro. Bronchial rings were sham
(control) or passively sensitized overnight by using sera from donors
demonstrating sensitivity to Dermatophagoides farinae and having immunoglobulin E (IgE)
concentrations of 2,600 ± 200 U/ml. Tissues were fixed
isometrically to force transducers to measure responses to electrical
field stimulation (EFS) and quick stretch (QS). The myogenic response
to QS was normalized to the maximal response to EFS (%EFS). The
myogenic response of sensitized BSM was 47.9 ± 10.9 %EFS to a QS
of ~6.5% optimal length (Lo);
sham-sensitized tissues had a myogenic response of 13.5 ± 6.4 %EFS
(P = 0.012 vs. passively sensitized).
A QS of ~13% Lo in sensitized
BSM caused a response of 82.8 ± 20.9 %EFS; sham-sensitized tissues
developed a response of 38.2 ± 17.3 %EFS
(P = 0.004). BSM incubated with serum
from nonallergic donors did not demonstrate increased QS response (4.6 ± 1.4 %EFS, P = not significant
vs. tissue exposed to atopic sera). However, tissues incubated in sera
from nonatopic donors supplemented with hapten-specific chimeric IgE
(JW8) demonstrated augmented myogenic response to QS of ~6.5% Lo (21.9 ± 6.2 %EFS, P = 0.027 vs. nonatopic
sera alone). We demonstrate that passive sensitization of human BSM
preparations causes induction and augmentation of myogenic contractions
to QS; this hyperresponsiveness corresponds to the IgE concentration in
sensitizing sera.
airway smooth muscle; bronchial smooth muscle; quick stretch; electrical field stimulation; serum immunoglobulin E
This article has been cited by other articles:
|
|
 |
|
  J. M. Hernandez, G. Cox, and L. J. Janssen Involvement of the Neurokinin-2 Receptor in Airway Smooth Muscle Stretch-Activated Contractions Assessed in Perfused Intact Bovine Bronchial Segments J. Pharmacol. Exp. Ther., November 1, 2008; 327(2): 503 - 510. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Baroffio, E. Crimi, and V. Brusasco Review: Airway smooth muscle as a model for new investigative drugs in asthma Therapeutic Advances in Respiratory Disease, June 1, 2008; 2(3): 129 - 139. [Abstract] [PDF]
|
|
|
|
 |
|
 R. W. Mitchell, M. L. Dowell, J. Solway, and O. J. Lakser Force Fluctuation induced Relengthening of Acetylcholine-contracted Airway Smooth Muscle Proceedings of the ATS, January 1, 2008; 5(1): 68 - 72. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. S. An, T. R. Bai, J. H. T. Bates, J. L. Black, R. H. Brown, V. Brusasco, P. Chitano, L. Deng, M. Dowell, D. H. Eidelman, et al. Airway smooth muscle dynamics: a common pathway of airway obstruction in asthma Eur. Respir. J., May 1, 2007; 29(5): 834 - 860. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. D. Mentzelopoulos, C. Roussos, and S. G. Zakynthinos Prone position improves expiratory airway mechanics in severe chronic bronchitis Eur. Respir. J., February 1, 2005; 25(2): 259 - 268. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 V. Brusasco and R. Pellegrino Invited Review: Complexity of factors modulating airway narrowing in vivo: relevance to assessment of airway hyperresponsiveness J Appl Physiol, September 1, 2003; 95(3): 1305 - 1313. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. J. Fernandes, R. W. Mitchell, O. Lakser, M. Dowell, A. G. Stewart, and J. Solway Invited Review: Do inflammatory mediators influence the contribution of airway smooth muscle contraction to airway hyperresponsiveness in asthma? J Appl Physiol, August 1, 2003; 95(2): 844 - 853. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Crimi, R. Pellegrino, M. Milanese, and V. Brusasco Deep breaths, methacholine, and airway narrowing in healthy and mild asthmatic subjects J Appl Physiol, October 1, 2002; 93(4): 1384 - 1390. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. SOLWAY What Makes the Airways Contract Abnormally? Is It Inflammation? Am. J. Respir. Crit. Care Med., March 1, 2000; 161(3): S164 - 167. [Full Text] [PDF]
|
|
|
|
|
|
J. L. BLACK and P. R. A. JOHNSON What Determines Asthma Phenotype? Is It the Interaction between Allergy and the Smooth Muscle? Am. J. Respir. Crit. Care Med., March 1, 2000; 161(3): S207 - 210. [Full Text] [PDF]
|
|
|
|
|
|
E. ROUX, J.-M. HYVELIN, J.-P. SAVINEAU, and R. MARTHAN Human Isolated Airway Contraction . Interaction between Air Pollutants and Passive Sensitization Am. J. Respir. Crit. Care Med., August 1, 1999; 160(2): 439 - 445. [Abstract] [Full Text]
|
|
|
|
|
|
H. W. Mitchell, D. J. Turner, P. R. Gray, and P. K. McFawn Compliance and stability of the bronchial wall in a model of allergen-induced lung inflammation J Appl Physiol, March 1, 1999; 86(3): 932 - 937. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. SONG, E. CRIMI, M. MILANESE, J. DUAN, K. REHDER, and V. BRUSASCO Anti-inflammatory Agents and Allergen-induced beta 2-Receptor Dysfunction in Isolated Human Bronchi Am. J. Respir. Crit. Care Med., December 1, 1998; 158(6): 1809 - 1814. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. F. RABE Mechanisms of Immune Sensitization of Human Bronchus Am. J. Respir. Crit. Care Med., November 1, 1998; 158(2007): S161 - S170. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Visit Other APS Journals Online |
