Journal of Applied Physiology AJP: Cell Physiology
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J Appl Physiol 83: 652-660, 1997;
8750-7587/97 $5.00
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Journal of Applied Physiology
Vol. 83, No. 2, pp. 652-660, August 1997
EXERCISE AND MUSCLE

Effect of inotropic interventions on contraction and Ca2+ transients in the human heart

Klara Brixius, Marcus Pietsch, Susanne Hoischen, Jochen Müller-Ehmsen, and Robert H. G. Schwinger

Medizinische Klinik III, Universität zu Köln, D-50924 Cologne, Germany

Received 29 October 1996; accepted in final form 25 March 1997.

Brixius, Klara, Marcus Pietsch, Susanne Hoischen, Jochen Müller-Ehmsen, and Robert H. G. Schwinger. Effect of inotropic interventions on contraction and on Ca2+ transients in the human heart. J. Appl. Physiol. 83(2): 652-660, 1997.---The present study investigated the influences of inotropic intervention on the intracellular Ca2+ transient {intracellular Ca2+ concentration ([Ca2+]i)} and contractile twitch. Isometric twitch and [Ca2+]i (fura 2 ratio method) were measured simultaneously (1 Hz, 37°C) after stimulation with Ca2+ (0.9-3.2 mM), the cardiac glycoside ouabain (Oua; 0.1 µM), the beta 1- and beta 2-adrenoceptor-agonist isoprenaline (Iso; 1-10 nM), and the Ca2+ sensitizer EMD-57033 (30 µM) by using isolated human nonfailing right auricular trabeculae (n = 19). Inotropic interventions increased force of contraction and peak rate of tension rise (+T) significantly. Only Iso stimulated peak rate of tension decay (-T) higher than +T (P < 0.05), thereby reducing time of contraction (Ttwitch). EMD-57033 increased +T more effectively than -T and prolonged Ttwitch (P < 0.05). Ca2+, Oua, and Iso, but not EMD-57033, increased systolic Ca2+. Diastolic Ca2+ increased after stimulation with Oua or Ca2+, but not in the presence of EMD-57033. Iso shortened the Ca2+ transient and did not influence diastolic Ca2+. In conclusion, positive inotropic agents differently affect force and [Ca2+]i depending on their mode of action. Inotropic interventions influence diastolic Ca2+ and thus may be less advantageous in a situation with altered intracellular Ca2+ homeostasis (e.g., heart failure due to dilated cardiomyopathy).

fura 2; heart failure; isoprenaline; ouabain; EMD-57033

0161-7567/97 $5.00 Copyright © 1997 the American Physiological Society




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