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J Appl Physiol 83: 74-81, 1997;
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Journal of Applied Physiology
Vol. 83, No. 1, pp. 74-81, July 1997
SYSTEMIC CIRCULATION AND FLUID BALANCE

Purified ACE attenuates smokeless tobacco-induced increase in macromolecular efflux from the oral mucosa

Xiao-Pei Gao, Hideyuki Suzuki, Christopher O. Olopade, Sergei Pakhlevaniants, and Israel Rubinstein

Department of Medicine, University of Illinois at Chicago, and West Side Department of Veterans Affairs Medical Center, Chicago, Illinois 60612

Received 8 July 1996; accepted in final form 7 March 1997.

Gao, Xiao-pei, Hideyuki Suzuki, Christopher O. Olopade, Sergei Pakhlevaniants, and Israel Rubinstein. Purified ACE attenuates smokeless tobacco-induced increase in macromolecular efflux from the oral mucosa. J. Appl. Physiol. 83(1): 74-81, 1997.---The purpose of this study was to determine whether purified angiotensin I-converting enzyme (ACE) attenuates smokeless tobacco extract (STE)-induced increase in macromolecular efflux from the in situ oral mucosa. By using intravital microscopy, we found that suffusion of an aqueous extract of smokeless tobacco elicited significant concentration-dependent leaky site formation and increase in clearance of fluorescein isothiocyanate-labeled dextran (mol mass, 70 kDa) from the hamster cheek pouch (P < 0.05). Suffusion of purified rabbit lung ACE significantly attenuated these responses in a concentration-dependent fashion (P < 0.05). These effects were specific because purified ACE also significantly attenuated the increase in macromolecular efflux elicited by bradykinin, which is produced in the cheek pouch during suffusion of STE, but did not attenuate the increase elicted by adenosine. Moreover, suffusion of heat-inactivated purified ACE and purified superoxide dismutase had no significant effects on STE- and bradykinin-induced responses. Collectively, these data suggest that exogenous ACE attenuates STE-induced increase in macromolecular efflux from the in situ oral mucosa, in part, by promoting local bradykinin catabolism.

inflammation; microcirculation; plasma exudation; bradykinin; angiotensin-I converting enzyme; hamster

0161-7567/97 $5.00 Copyright © 1997 the American Physiological Society




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