Journal of Applied Physiology Ad Instruments
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

J Appl Physiol 83: 323, 1997;
8750-7587/97 $5.00
This Article
Right arrow Full Text Free
Right arrow Full Text (PDF) Free
Right arrow Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Pickar, J. G.
Right arrow Articles by Musch, T. I.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Pickar, J. G.
Right arrow Articles by Musch, T. I.

Journal of Applied Physiology
Vol. 83, No. 1, pp. 323-323, July 1997
EXERCISE AND MUSCLE

RAPID COMMUNICATION

Decreased [3H]ouabain binding sites in skeletal muscle of rats with chronic heart failure

Joel G. Pickar1, John P. Mattson1, Steve Lloyd2, and Timothy I. Musch1,2

Departments of 1 Anatomy and Physiology and 2 Kinesiology, Kansas State University, Manhattan, Kansas 66506

Received 31 December 1996; accepted in final form 14 March 1997.

Pickar, Joel G., John P. Mattson, Steve Lloyd, and Timothy I. Musch. Decreased [3H]ouabain binding sites in skeletal muscle of rats with chronic heart failure. J. Appl. Physiol. 83(1): 323-329, 1997.---Abnormalities intrinsic to skeletal muscle are thought to contribute to decrements in exercise capacity found in individuals with chronic heart failure (CHF). Na+-K+-adenosinetriphosphatase (the Na+ pump) is essential for maintaining muscle excitability and contractility. Therefore, we investigated the possibility that the number and affinity of Na+ pumps in locomotor muscles of rats with CHF are decreased. Myocardial infarction (MI) was induced in 8 rats, and a sham operation was performed in 12 rats. The degree of CHF was assessed ~180 days after surgery. Soleus and plantaris muscles were harvested, and Na+ pumps were quantified by using a [3H]ouabain binding assay. At the time of muscle harvest, MI and sham-operated rats were similar in age (458 ± 54 vs. 447 ± 34 days old, respectively). Compared with their sham-operated counterparts, MI rats had a significant amount of heart failure, right ventricular-to-body weight ratio was greater (48%), and the presence of pulmonary congestion was suggested by an elevated lung-to-body weight ratio (29%). Left ventricular end-diastolic pressure was significantly increased in the MI rats (11 ± 1 mmHg) compared with the sham-operated controls (1 ± 1 mmHg). In addition, mean arterial blood pressure was lower in the MI rats compared with their control counterparts. [3H]ouabain binding sites were reduced 18% in soleus muscle (136 ± 12 vs. 175 ± 13 pmol/g wet wt, MI vs. sham, respectively) and 22% in plantaris muscle (119 ± 12 vs. 147 ± 8 pmol/g wet wt, MI vs. sham, respectively). The affinity of these [3H]ouabain binding sites was similar for the two groups. The relationship between the reduction in Na+ pump number and the reduced exercise capacity in individuals with CHF remains to be determined.

sodium; pump; sodium-potassium-adenosinetriphosphatase

0161-7567/97 $5.00 Copyright © 1997 the American Physiological Society




This article has been cited by other articles:


Home page
 J. Appl. Physiol.Home page
D. J. Barr, H. J. Green, D. S. Lounsbury, J. W. E. Rush, and J. Ouyang
Na+-K+-ATPase properties in rat heart and skeletal muscle 3 mo after coronary artery ligation
J Appl Physiol, August 1, 2005; 99(2): 656 - 664.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
J. R. Fowles, H. J. Green, and J. Ouyang
Na+-K+-ATPase in rat skeletal muscle: content, isoform, and activity characteristics
J Appl Physiol, January 1, 2004; 96(1): 316 - 326.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. Rev.Home page
T. CLAUSEN
Na+-K+ Pump Regulation and Skeletal Muscle Contractility
Physiol Rev, October 1, 2003; 83(4): 1269 - 1324.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
B. Helwig, K. M. Schreurs, J. Hansen, K. S. Hageman, M. G. Zbreski, R. M. McAllister, K. E. Mitchell, and T. I. Musch
Training-induced changes in skeletal muscle Na+-K+ pump number and isoform expression in rats with chronic heart failure
J Appl Physiol, June 1, 2003; 94(6): 2225 - 2236.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
T. I. Musch, S. Wolfram, K. S. Hageman, and J. G. Pickar
Skeletal muscle ouabain binding sites are reduced in rats with chronic heart failure
J Appl Physiol, June 1, 2002; 92(6): 2326 - 2334.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
H. Green, B. Roy, S. Grant, M. Burnett, R. Tupling, C. Otto, A. Pipe, and D. McKenzie
Downregulation in muscle Na+-K+-ATPase following a 21-day expedition to 6,194 m
J Appl Physiol, February 1, 2000; 88(2): 634 - 640.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
H. Green, J. MacDougall, M. Tarnopolsky, and N. L. Melissa
Downregulation of Na+-K+-ATPase pumps in skeletal muscle with training in normobaric hypoxia
J Appl Physiol, May 1, 1999; 86(5): 1745 - 1748.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online