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J Appl Physiol 82: 1995-2002, 1997;
8750-7587/97 $5.00
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Journal of Applied Physiology
Vol. 82, No. 6, pp. 1995-2002, June 1997
SYSTEMIC CIRCULATION AND FLUID BALANCE

Blood volume and cardiac index in rats after exchange transfusion with hemoglobin-based oxygen carriers

Russell Migita, Armando Gonzales, Maria L. Gonzales, Kim D. Vandegriff, and Robert M. Winslow

Department of Medicine, School of Medicine, University of California, San Diego, and Department of Veterans Affairs Medical Center, San Diego, California 92161

Received 30 July 1996; accepted in final form 29 January 1997.

Migita, Russell, Armando Gonzales, Maria L. Gonzales, Kim D. Vandegriff, and Robert M. Winslow. Blood volume and cardiac index in rats after exchange transfusion with hemoglobin-based oxygen carriers. J. Appl. Physiol. 82(6): 1995-2002, 1997.---We have measured plasma volume and cardiac index in rats after 50% isovolemic exchange transfusion with human hemoglobin cross-linked between the alpha -chains with bis(3,5-dibromosalicyl)fumarate (alpha alpha Hb) and with bovine hemoglobin modified with polyethylene glycol (PEGHb). alpha alpha Hb and PEGHb differ in colloid osmotic pressure (23.4 and 118.0 Torr, respectively), oxygen affinity (oxygen half-saturation pressure of hemoglobin = 30.0 and 10.2 Torr, respectively), viscosity (1.00 and 3.39 cP, respectively), and molecular weight (64,400 and 105,000, respectively). Plasma volume was measured by Evans blue dye dilution modified for interference by plasma hemoglobin. Blood volumes in PEGHb-treated animals were significantly elevated (74.0 ± 3.5 ml/kg) compared with animals treated with alpha alpha Hb (49.0 ± 1.2 ml/kg) or Ringer lactate (48.0 ± 2.0 ml/kg) or with controls (58.2 ± 1.9 ml/kg). Heart rate reduction after alpha alpha Hb exchange is opposite to that expected with blood volume contraction, suggesting that alpha alpha Hb may have a direct myocardial depressant action. The apparently slow elimination of PEGHb during the 2 h after its injection is a consequence of plasma volume expansion: when absolute hemoglobin (concentration × plasma volume) is compared for PEGHb and alpha alpha Hb, no difference in their elimination rates is found. These studies emphasize the need to understand blood volume regulation when the effects of cell-free hemoglobin on hemodynamic measurements are evaluated.

alpha alpha hemoglobin; cardiac output; polyethylene glycol-modified hemoglobin; blood substitutes; colloid osmotic pressure; plasma volume; Evans blue dye; bovine hemoglobin

0161-7567/97 $5.00 Copyright © 1997 the American Physiological Society




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