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J Appl Physiol 82: 1926-1931, 1997;
8750-7587/97 $5.00
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Journal of Applied Physiology
Vol. 82, No. 6, pp. 1926-1931, June 1997
METABOLISM

Exercise-induced changes in beta -adrenergic-receptor mRNA level measured by competitive RT-PCR

Nobuharu Fujii1, Takeshi Shibata1, Sachiko Homma2, Haruo Ikegami3, Kazuo Murakami1, and Hitoshi Miyazaki1

1 Gene Experiment Center, Institute of Applied Biochemistry, University of Tsukuba, Tsukuba-City 305; 2 Research Institute of Physical Fitness, Japan Women's College of Physical Education, Tokyo 157; and 3 Department of Physical Education, International Budo University, Katsuura 299-52, Japan

Received 4 March 1996; accepted in final form 12 February 1997.

Fujii, Nobuharu, Takeshi Shibata, Sachiko Homma, Haruo Ikegami, Kazuo Murakami, and Hitoshi Miyazaki. Exercise-induced changes in beta -adrenergic-receptor mRNA level measured by competitive RT-PCR. J. Appl. Physiol. 82(6): 1926-1931, 1997.---Competitive reverse transcription-polymerase chain reaction (RT-PCR) analysis was used to clarify whether dynamic exercise-induced increases in beta -adrenergic-receptor (beta -AR) number in human lymphocytes are accompanied by increases in the beta -AR mRNA level. Sixteen healthy subjects performed cycle ergometry until exhaustion. Before and immediately after exercise, peripheral blood was drawn from a forearm vein for preparation of lymphocytes. Both the beta -AR mRNA level and the beta -AR number were significantly increased by exercise. The changes in beta -AR mRNA level and beta -AR number were significantly correlated (r = 0.63, P < 0.01). This finding suggests that a rapid increase in beta -AR mRNA level might be an early adaptive response of the sympathetic nervous system to dynamic exercise. In vitro incubation of lymphocytes with epinephrine had no effect on beta -AR mRNA levels, nor did adenosine 3',5'-cyclic monophosphate, protein kinase C, or intracellular Ca2+ increase the beta -AR mRNA level in vitro. Therefore, it appears that other mechanisms underlie the exercise-induced elevation of beta -AR mRNA levels in human lymphocytes.

reverse transcription-polymerase chain reaction; upregulation; catecholamine; lymphocyte

0161-7567/97 $5.00 Copyright © 1997 the American Physiological Society




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