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University of British Columbia Pulmonary Research Laboratory, St. Paul's Hospital, Vancouver, British Columbia, Canada V6Z 1Y6
Received 30 September 1996; accepted in final form 22 January 1997.
Kitagawa, Yuko, Stephan F. Van Eeden, Darlene M. Redenbach,
Maleki Daya, Blair A. M. Walker, Maria E. Klut, Barry R. Wiggs, and
James C. Hogg. Effect of mechanical deformation on structure and
function of polymorphonuclear leukocytes. J. Appl.
Physiol. 82(5): 1397-1405, 1997.
The present
studies were designed to test the hypothesis that mechanical
deformation of polymorphonuclear leukocytes (PMN) leads to functional
changes that might influence their transit in the pulmonary
capillaries. Human leukocytes were passed through 5- or 3-µm-pore
polycarbonate filters under controlled conditions. Morphometric
analysis showed that the majority of PMN were deformed and that this
deformation persisted longer after filtration through 3-µm filters
than through 5-µm filters (P < 0.05) but did not result in the cytoskeletal polarization
characteristic of migrating cells. Flow cytometric studies of the
filtered PMN showed that there was a transient increase in the
cytosolic free Ca2+ concentration
after both 3- and 5-µm filtration (P < 0.01) with an increase in F-actin content after 3-µm filtration
(P < 0.05). Although
L-selectin expression on PMN was
not changed by either 5- or 3-µm filtration, CD18 and CD11b were
increased by 3-µm filtration (P < 0.05). Priming of the PMN with
N-formyl-methionyl-leucyl-phenylalanine (0.5 nM) before filtration resulted in an increase of CD11b by both 5 (P < 0.05)- and 3-µm
(P < 0.01) filtration. Neither 5- nor 3-µm filtration induced hydrogen peroxide production. We conclude that mechanical deformation of PMN, similar to what occurs in the
pulmonary microvessels, induces both structural and functional changes
in the cells, which might influence their passage through the pulmonary
capillary bed.
neutrophils; deformability; F-actin; adhesion molecules
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