Journal of Applied Physiology
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J Appl Physiol 82: 1145-1153, 1997;
8750-7587/97 $5.00
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Journal of Applied Physiology
Vol. 82, No. 4, pp. 1145-1153, April 1997
EXERCISE AND MUSCLE

Metabolic and phenotypic adaptations of diaphragm muscle fibers with inactivation

Wen-Zhi Zhan, Hirofumi Miyata, Y. S. Prakash, and Gary C. Sieck

Departments of Anesthesiology and of Physiology and Biophysics, Mayo Foundation, Rochester, Minnesota 55905

Received 27 June 1996; accepted in final form 20 November 1996.

Zhan, Wen-Zhi, Hirofumi Miyata, Y. S. Prakash, and Gary C. Sieck. Metabolic and phenotypic adaptations of diaphragm muscle fibers with inactivation. J. Appl. Physiol. 82(4):1145-1153, 1997.---We hypothesized that metabolic adaptations to muscle inactivity are most pronounced when neurotrophic influence is disrupted. In rat diaphragm muscle (Diam), 2 wk of unilateral denervation or tetrodotoxin nerve blockade resulted in a reduction in succinate dehydrogenase (SDH) activity of type I, IIa, and IIx fibers (~50, 70, and 24%, respectively) and a decrease in SDH variability among fibers (~63%). In contrast, inactivity induced by spinal cord hemisection at C2 (ST) resulted in much less change in SDH activity of type I and IIa fibers (~27 and 24%, respectively) and only an ~30% reduction in SDH variability among fibers. Actomyosin adenosinetriphosphatase (ATPase) activities of type I, IIx, and IIb fibers in denervated and tetrodotoxin-treated Diam were reduced by ~20, 45, and 60%, respectively, and actomyosin ATPase variability among fibers was ~60% lower. In contrast, only actomyosin ATPase activity of type IIb fibers was reduced (~20%) in ST Diam. These results suggest that disruption of neurotrophic influence has a greater impact on muscle fiber metabolic properties than inactivity per se.

spinal hemisection; tetrodotoxin; denervation; muscle fiber; succinate dehydrogenase activity; actomyosin adenosinetriphosphatase activity

0161-7567/97 $5.00 Copyright © 1997 the American Physiological Society




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