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Department of Medicine B, The Heart Centre, Rigshospitalet, National University Hospital, DK-2100 Copenhagen, Denmark
Received 12 March 1996; accepted in final form 25 November 1996.
Bundgaard, Henning, Thomas A. Schmidt, Jim S. Larsen, and
Keld Kjeldsen. K+
supplementation increases muscle
[Na+-K+-ATPase]
and improves extrarenal K+
homeostasis in rats. J. Appl. Physiol.
82(4): 1136-1144, 1997.
Effects of
K+ supplementation (~200 mmol
KCl/100 g chow) on plasma K+,
K+ content, and
Na+-K+-adeonsinetriphosphatase
(ATPase) concentration
([Na+-K+-ATPase])
in skeletal muscles as well as on extrarenal
K+ clearance were evaluated in
rats. After 2 days of K+
supplementation, hyperkalemia prevailed
(K+-supplemented vs.
weight-matched control animals) [5.1 ± 0.2 (SE) vs. 3.2 ± 0.1 mmol/l, P < 0.05, n = 5-6], and after 4 days
a significant increase in K+
content was observed in gastrocnemius muscle (104 ± 2 vs. 97 ± 1 µmol/g wet wt, P < 0.05, n = 5-6). After 7 days of
K+ supplementation, a significant
increase in
[3H]ouabain binding
site concentration (344 ± 5 vs. 239 ± 8 pmol/g wet wt,
P < 0.05, n = 4) was observed in gastrocnemius
muscle. After 2 wk, increases in plasma
K+,
K+ content, and
[3H]ouabain binding
site concentration in gastrocnemius muscle amounted to 40, 8, and 68%
(P < 0.05) above values observed in
weight-matched control animals, respectively. The latter change was
confirmed by K+-dependent
p-nitrophenyl phosphatase activity
measurements. Fasting for 1 day reduced plasma
K+ and
K+ content in gastrocnemius muscle
in rats that had been K+
supplemented for 2 wk by 3.1 ± 0.3 mmol/l
(P < 0.05, n = 5) and 15 ± 2 µmol/g wet wt
(P < 0.05, n = 5), respectively. After induction of anesthesia, arterial plasma K+
was measured during intravenous KCl infusion (0.75 mmol
KCl · 100 g body
wt
1 · h1).
The K+-supplemented fasted group
demonstrated a 42% (P < 0.05) lower plasma K+ rise, associated with a
significantly higher increase in
K+ content in gastrocnemius muscle
of 7 µmol/g wet wt (P < 0.05, n = 5) compared with their control
animals. In conclusion, K+
supplementation increases plasma
K+,
K+ content, and
[Na+-K+-ATPase]
in skeletal muscles and improves extrarenal
K+ clearance capacity.
plasma potassium; muscle potassium; potassium adaptation; sodium-potassium-adenosinetriphosphatase concentration
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