Journal of Applied Physiology Fuel your research with LabChart
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

J Appl Physiol 82: 1119-1125, 1997;
8750-7587/97 $5.00
This Article
Right arrow Full Text Free
Right arrow Full Text (PDF) Free
Right arrow Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Supinski, G. S.
Right arrow Articles by Dimarco, A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Supinski, G. S.
Right arrow Articles by Dimarco, A.

Journal of Applied Physiology
Vol. 82, No. 4, pp. 1119-1125, April 1997
EXERCISE AND MUSCLE

N-acetylcysteine administration alters the response to inspiratory loading in oxygen-supplemented rats

G. S. Supinski, D. Stofan, R. Ciufo, and A. Dimarco

Pulmonary Division, Department of Medicine, Case Western Reserve University, Cleveland 44106; and Metrohealth Medical Center, Cleveland, Ohio 44109

Received 26 September 1995; accepted in final form 15 November 1996.

Supinski, G. S., D. Stofan, R. Ciufo, and A. DiMarco. N-acetylcysteine administration alters the response to inspiratory loading in oxygen-supplemented rats. J. Appl. Physiol. 82(4): 1119-1125, 1997.---Based on recent studies, it has been suggested that free radicals are elaborated in the respiratory muscles during strenuous contractions and contribute to the development of muscle fatigue. If this theory is correct, then it should be possible to attenuate the development of diaphragm fatigue and/or delay the onset of respiratory failure during loaded breathing by administering a free radical scavenger. The purpose of the present experiment was, therefore, to examine the effect of N-acetylcysteine (NAC), a free radical scavenger and glutathione precursor, on the evolution of respiratory failure in decerebrate unanesthetized rats breathing against a large inspiratory resistive load. We compared the inspiratory volume and pressure generation over time in animals pretreated with either saline or NAC (150 mg/kg) and then loaded until respiratory arrest. After arrest, the diaphragm was excised, and samples were assayed for reduced (GSH) and oxidized glutathione. As a control, we also assessed respiratory function and glutathione concentrations in groups of nonloaded saline- and NAC-treated animals. We found that NAC-treated animals were able to tolerate loading better than the saline-treated group, maintaining higher inspiratory pressures and sustaining higher inspired volumes. Administration of NAC also increased the time that animals could tolerate loading before the development of respiratory arrest. In addition, although saline-treated loaded animals had significant reductions in diaphragmatic GSH levels compared with unloaded controls, the magnitude of this reduction was blunted by NAC administration (i.e., GSH averaged 965 ± 113, 568 ± 83, 907 ± 39, and 784 ± 61 nmol/g for unloaded-saline, loaded-saline, unloaded-NAC, and loaded-NAC groups, P < 0.05, with the value for the loaded-saline group lower than the values for the two unloaded groups; GSH for the loaded-NAC group was not different, however, from unloaded controls). These data demonstrate that administration of NAC, a free radical scavenger, slows the rate of development of respiratory failure during inspiratory resistive loading.

free radicals; skeletal muscle

0161-7567/97 $5.00 Copyright © 1997 the American Physiological Society




This article has been cited by other articles:


Home page
 J. Appl. Physiol.Home page
L. F. Ferreira, L. A. A. Gilliam, and M. B. Reid
L-2-Oxothiazolidine-4-carboxylate reverses glutathione oxidation and delays fatigue of skeletal muscle in vitro
J Appl Physiol, July 1, 2009; 107(1): 211 - 216.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. Rev.Home page
S. K. Powers and M. J. Jackson
Exercise-Induced Oxidative Stress: Cellular Mechanisms and Impact on Muscle Force Production
Physiol Rev, October 1, 2008; 88(4): 1243 - 1276.
[Abstract] [Full Text] [PDF]

Home page
 Biol Res NursHome page
C. A. Goodyear-Bruch, J. Jegathesan, R. L. Clancy, and J. D. Pierce
Apoptotic-Related Protein Expression in the Diaphragm and the Effect of Dopamine During Inspiratory Resistance Loading
Biol Res Nurs, April 1, 2008; 9(4): 293 - 300.
[Abstract] [PDF]

Home page
 J. Appl. Physiol.Home page
L. F. Ferreira and M. B. Reid
Muscle-derived ROS and thiol regulation in muscle fatigue
J Appl Physiol, March 1, 2008; 104(3): 853 - 860.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
J. A. Simpson and S. Iscoe
Cardiorespiratory failure in rat induced by severe inspiratory resistive loading
J Appl Physiol, April 1, 2007; 102(4): 1556 - 1564.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
T. Vassilakopoulos and S. N. A. Hussain
Ventilatory muscle activation and inflammation: cytokines, reactive oxygen species, and nitric oxide
J Appl Physiol, April 1, 2007; 102(4): 1687 - 1695.
[Abstract] [Full Text] [PDF]

Home page
 Exp PhysiolHome page
J. D. Pierce, C. Goodyear-Bruch, S. Hall, and R. L. Clancy
Effect of dopamine on rat diaphragm apoptosis and muscle performance
Exp Physiol, July 1, 2006; 91(4): 731 - 740.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
S. N. A. Hussain, G. Matar, E. Barreiro, M. Florian, M. Divangahi, and T. Vassilakopoulos
Modifications of proteins by 4-hydroxy-2-nonenal in the ventilatory muscles of rats
Am J Physiol Lung Cell Mol Physiol, May 1, 2006; 290(5): L996 - L1003.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
E. Barreiro, J. B. Galdiz, M. Marinan, F. J. Alvarez, S. N. A. Hussain, J. Gea, and on behalf of the ENIGMA in COPD project
Respiratory loading intensity and diaphragm oxidative stress: N-acetyl-cysteine effects
J Appl Physiol, February 1, 2006; 100(2): 555 - 563.
[Abstract] [Full Text] [PDF]

Home page
 Adv. Physiol. Educ.Home page
M. Kristensen and T. Hansen
Statistical analyses of repeated measures in physiological research: a tutorial
Advan Physiol Educ, March 1, 2004; 28(1): 2 - 14.
[Abstract] [Full Text] [PDF]

Home page
 J. Physiol.Home page
J. A. Simpson, J. Van Eyk, and S. Iscoe
Respiratory muscle injury, fatigue and serum skeletal troponin I in rat
J. Physiol., February 1, 2004; 554(3): 891 - 903.
[Abstract] [Full Text] [PDF]

Home page
 Biol Res NursHome page
J. D. Pierce, R. L. Clancy, N. Smith-Blair, and R. Kraft
Treatment and Prevention of Diaphragm Fatigue Using Low-Dose Dopamine
Biol Res Nurs, January 1, 2002; 3(3): 140 - 149.
[Abstract] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
T.-X. JIANG, W. DARLENE REID, and J. D. ROAD
Free Radical Scavengers and Diaphragm Injury Following Inspiratory Resistive Loading
Am. J. Respir. Crit. Care Med., October 1, 2001; 164(7): 1288 - 1294.
[Abstract] [Full Text] [PDF]

Home page
 ThoraxHome page
L. M A Heunks and P N R. Dekhuijzen
Respiratory muscle function and free radicals: from cell to COPD
Thorax, August 1, 2000; 55(8): 704 - 716.
[Full Text]

Home page
 J. Appl. Physiol.Home page
D. Nethery, L. A. Callahan, D. Stofan, R. Mattera, A DiMarco, and G. Supinski
PLA2 dependence of diaphragm mitochondrial formation of reactive oxygen species
J Appl Physiol, July 1, 2000; 89(1): 72 - 80.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
D. A. STOFAN, L. A. CALLAHAN, A. F. DiMARCO, D. E. NETHERY, and G. S. SUPINSKI
Modulation of Release of Reactive Oxygen Species by the Contracting Diaphragm
Am. J. Respir. Crit. Care Med., March 1, 2000; 161(3): 891 - 898.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
G. Supinski, D. Nethery, D. Stofan, and A. DiMarco
Extracellular calcium modulates generation of reactive oxygen species by the contracting diaphragm
J Appl Physiol, December 1, 1999; 87(6): 2177 - 2185.
[Abstract] [Full Text] [PDF]

Home page
 Exp. Biol. Med.Home page
T. L. Clanton, L. Zuo, and P. Klawitter
Oxidants and Skeletal Muscle Function: Physiologic and Pathophysiologic Implications
Experimental Biology and Medicine, December 1, 1999; 222(3): 253 - 262.
[Abstract] [Full Text]

Home page
 J. Appl. Physiol.Home page
L. C. Lands, V. L. Grey, and A. A. Smountas
Effect of supplementation with a cysteine donor on muscular performance
J Appl Physiol, October 1, 1999; 87(4): 1381 - 1385.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
G. Supinski, D. Nethery, D. Stofan, L. Szweda, and A. DiMarco
Oxypurinol administration fails to prevent free radical-mediated lipid peroxidation during loaded breathing
J Appl Physiol, September 1, 1999; 87(3): 1123 - 1131.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
D. Nethery, D. Stofan, L. Callahan, A. DiMarco, and G. Supinski
Formation of reactive oxygen species by the contracting diaphragm is PLA2 dependent
J Appl Physiol, August 1, 1999; 87(2): 792 - 800.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online