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Department of Clinical Chemistry, University of Helsinki, SF-00290 Helsinki, Finland
Received 24 July 1996; accepted in final form 4 November 1996.
Jänkälä, Heidi, Veli-Pekka Harjola, Niels
Erik Petersen, and Matti Härkönen. Myosin heavy chain
mRNA transform to faster isoforms in immobilized skeletal muscle: a
quantitative PCR study. J. Appl.
Physiol. 82(3): 977-982, 1997.
A quantitative polymerase chain reaction (PCR) method was used to measure the quantities of type I, IIa, IIx, and IIb myosin heavy chain (MHC) mRNA
in total RNA preparations of the soleus, gastrocnemius, and plantaris
muscles of normal and hindlimb-immobilized rats. Type IIx and even type
IIb MHC mRNA were demonstrated at extremely low levels in normal
soleus, 2.1 ± 0.4 × 105
and 5.0 ± 0.2 × 105
molecules of mRNA per microgram total RNA, respectively. Immobilization for 1 wk significantly altered the gene expression of MHC isoforms. In
soleus, both type IIx and IIb MHC genes became significantly upregulated, 24-fold (P < 0.005) and 2.6-fold (P < 0.05),
respectively. In gastrocnemius, the level of type IIa MHC mRNA
decreased by 51% (P < 0.01) and the
level of type IIx MHC mRNA increased by 140%
(P < 0.05). In plantaris, the level
of type IIa MHC mRNA decreased by 58%
(P < 0.005). In conclusion,
immobilization changed the MHC mRNA profile in three different types of
skeletal muscle toward faster isoforms. The quantitative results permit
reliable evaluation of changes in mRNA levels.
immobilization; number of myosin heavy chain mRNA molecules; quantitative reverse transcription-polymerase chain reaction
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