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Muscle Biology Laboratory, Texas A&M University, College Station, Texas 77843, and Muscular Function Laboratory, Virginia Polytechnic Institute, Blacksburg, Virginia 24061
Received 22 May 1996; accepted in final form 2 August 1996.
Warren III, Gordon L., Jay H. Williams, Christopher W. Ward,
Hideki Matoba, Christopher P. Ingalls, Karl M. Hermann, and R. B. Armstrong. Decreased contraction economy in mouse EDL muscle
injured by eccentric contractions. J. Appl.
Physiol. 81(6): 2555-2564, 1996.
The objective of
this study was to find out whether basal and/or active energy
metabolism are altered in isolated mouse extensor digitorum longus
muscle injured by eccentric (Ecc) contractions. Measurements of basal
O2 consumption and isometric tetanus O2 recovery cost were made
at 25°C on muscles that had done either 10 Ecc, 10 isometric (Iso),
or no contractions (No). In parallel experiments, rates of lactate and
pyruvate production were measured to estimate the anaerobic
contribution. Basal O2 consumption
was unaffected by the type of protocol performed
(P = 0.07). However, the tetanus
O2 cost per force-time integral was elevated by 30-36% for the Ecc protocol muscles over that for
the Iso and No protocol muscles. When including the increased lactate
production by the Ecc protocol muscles, the total energetic cost per
force-time integral was 53% higher than that for the Iso protocol
muscles [2.35 ± 0.17 vs. 1.54 ± 0.18 µmol
O2/(N · m · s)].
The decreased economy was attributed to two factors. First, in skinned
fibers isolated from the injured muscles, the ratio of maximal
actomyosin adenosinetriphosphatase activity to force production was up
by 37.5%, suggesting uncoupling of ATP hydrolysis from force
production. Second, increased reliance on anaerobic metabolism along
with the fluorescent microscopic study of mitochondrial membrane
potential and histochemical study of ATP synthase suggested an
uncoupling of oxidative phosphorylation in the injured muscles.
oxygen consumption; lactate release; actomyosin adenosinetriphosphatase; confocal microscopy; ATP synthase
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