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J Appl Physiol 81: 2509-2516, 1996;
8750-7587/96 $5.00
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Journal of Applied Physiology
Vol. 81, No. 6, pp. 2509-2516, December 1996
EXERCISE AND MUSCLE

The relationships among IGF-1, DNA content, and protein accumulation during skeletal muscle hypertrophy

G. R. Adams and F. Haddad

Department of Physiology and Biophysics, University of California, Irvine, California 92697

Received 23 April 1996; accepted in final form 13 August 1996.

Adams, G. R., and F. Haddad. The relationships among IGF-1, DNA content, and protein accumulation during skeletal muscle hypertrophy. J. Appl. Physiol. 81(6): 2509-2516, 1996.---Insulin-like growth factor-1 (IGF-1) is known to have anabolic effects on skeletal muscle cells. This study examined the time course of muscle hypertrophy and associated IGF-1 peptide and mRNA expression. Data were collected at 3, 7, 14, and 28 days after surgical removal of synergistic muscles of both normal and hypophysectomized (HX) animals. Overloading increased the plantaris (Plant) mass, myofiber size, and protein-to-body weight ratio in both groups (normal and HX; P < 0.05). Muscle IGF-1 peptide levels peaked at 3 (normal) and 7 (HX) days of overloading with maximum 4.1-fold (normal) and 6.2-fold (HX) increases. Increases in muscle IGF-1 preceded the hypertrophic response. Total DNA content of the overloaded Plant increased in both groups. There was a strong positive relationship between IGF-1 peptide and DNA content in the overloaded Plant from both groups. These results indicate that 1) the muscles from rats with both normal and severely depressed systemic levels of IGF-1 respond to functional overload with an increase in local IGF-1 expression and 2) this elevated IGF-1 may be contributing to the hypertrophy response, possibly via the mobilization of satellite cells to provide increases in muscle DNA.

growth factors; somatomedin; hypophysectomy; functional overload; ribonuclease protection assay

0161-7567/96 $5.00 Copyright © 1996 the American Physiological Society




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