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J Appl Physiol 81: 1965-1972, 1996;
8750-7587/96 $5.00
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Journal of Applied Physiology
Vol. 81, No. 5, pp. 1965-1972, November 1996
EXERCISE AND MUSCLE

Hypothyroidism alters diaphragm muscle development

Gary C. Sieck, Louise E. Wilson, Bruce D. Johnson, and Wen-Zhi Zhan

Departments of Anesthesiology and Physiology and Biophysics, Mayo Clinic and Foundation, Rochester, Minnesota 55905

Received 6 February 1996; accepted in final form 28 June 1996.

Sieck, Gary C., Louise E. Wilson, Bruce D. Johnson, and Wen-Zhi Zhan. Hypothyroidism alters diaphragm muscle development. J. Appl. Physiol. 81(5): 1965-1972, 1996.---The impact of hypothyroidism (Hyp) on myosin heavy chain (MHC) isoform expression, maximum specific force (Po), fatigability, and maximum unloaded shortening velocity (Vo) was determined in the rat diaphragm muscle (Dia) at 0, 7, 14, 21, and 28 days of age. Hyp was induced by treating pregnant rats with 6-n-propyl-2-thiouracil (0.05% in drinking water) beginning at gestational day 10 and was confirmed by reduced plasma levels of 3,5,3'-triiodothyronine and thyroxine. MHC isoforms were separated on sodium dodecyl sulfate-polyacrylamide gel electrophoresis gels and analyzed by densitometry. Isometric Po and fatigue resistance of the Dia were measured in vitro at 26°C, and Vo was determined at 15°C with the slack test. Compared with control muscles, expression of MHC-slow was higher and expression of adult fast MHC isoforms was lower in Hyp Dia at all ages. The neonatal isoform of MHC continued to be expressed in the Hyp Dia until day 28. At each age, Po and fatigability were reduced and Vo was slower in the Hyp Dia. We conclude that Hyp-induced alterations in MHC isoform expression do not fully predict the changes in Dia contractile properties.

myosin heavy chain; contractile properties; maturation; specific force; fatigue; shortening velocity

0161-7567/96 $5.00 Copyright © 1996 the American Physiological Society




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