| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Journal of Applied Physiology, Vol 81, Issue 3 1150-1155, Copyright © 1996 by American Physiological Society
ARTICLES |
P. S. Wendling, S. J. Peters, G. J. Heigenhauser and L. L. Spriet
Department of Human Biology and Nutritional Sciences, University of Guelph, Ontario, Canada.
The variability of the triacylglycerol store in human skeletal muscle (TGm) was examined using the needle biopsy technique. In 13 subjects, three biopsies were sampled from the vastus lateralis muscle of one leg at rest and after 90 min of cycling at 65% of maximal O2 uptake on one or two occasions. Visible fat and blood were removed before the samples were frozen, and remaining blood, connective tissue, and fat were removed from freeze-dried fiber bundles. TGm content was measured in two aliquots of powdered muscle from each biopsy. Within-biopsy variability was low at 6%. Despite precautions, many biopsies from inactive subjects were contaminated with adipose tissue. The TGm between-biopsy coefficient of variation (CV) was 23.5 +/- 14.6% (SD, n = 24) for rest and exercise time points where three noncontaminated biopsies existed. The between-biopsy variability at rest (19.8 +/- 7.9%, n = 10) was not significantly different from that at exercise (26.1 +/- 17.4%, n = 14). The muscle glycogen between-biopsy CV for rest and exercise time points was 10.0 +/- 10.3%. The resting TGm content was 26.3 +/- 4.3 mmol/kg dry muscle, and the net utilization during the 90 min of exercise was less than the between-biopsy variability. It is concluded that the TGm store measured in repeated biopsies of human skeletal muscle is variable, with a CV of 20-26%. Therefore, because of this high variability, only changes greater than approximately 24% of resting TGm content may be considered meaningful.
This article has been cited by other articles:
|
|
 |
|
  M. C. Devries, S. A. Lowther, A. W. Glover, M. J. Hamadeh, and M. A. Tarnopolsky IMCL area density, but not IMCL utilization, is higher in women during moderate-intensity endurance exercise, compared with men Am J Physiol Regulatory Integrative Comp Physiol, December 1, 2007; 293(6): R2336 - R2342. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Tarnopolsky, S. Phillips, G. Parise, A. Varbanov, J. DeMuth, P. Stevens, A. Qu, F. Wang, and R. Isfort Gene Expression, Fiber Type, and Strength Are Similar Between Left and Right Legs in Older Adults J. Gerontol. A Biol. Sci. Med. Sci., October 1, 2007; 62(10): 1088 - 1095. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M.-H. Cui, J.-H. Hwang, V. Tomuta, Z. Dong, and D. T. Stein Cross contamination of intramyocellular lipid signals through loss of bulk magnetic susceptibility effect differences in human muscle using 1H-MRSI at 4 T J Appl Physiol, October 1, 2007; 103(4): 1290 - 1298. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 X. Zhou, D. Li, J. Yin, J. Ni, B. Dong, J. Zhang, and M. Du CLA differently regulates adipogenesis in stromal vascular cells from porcine subcutaneous adipose and skeletal muscle J. Lipid Res., August 1, 2007; 48(8): 1701 - 1709. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. De Bock, T. Dresselaers, B. Kiens, E. A. Richter, P. Van Hecke, and P. Hespel Evaluation of intramyocellular lipid breakdown during exercise by biochemical assay, NMR spectroscopy, and Oil Red O staining Am J Physiol Endocrinol Metab, July 1, 2007; 293(1): E428 - E434. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Stellingwerff, H. Boon, R. A. M. Jonkers, J. M. Senden, L. L. Spriet, R. Koopman, and L. J. C. van Loon Significant intramyocellular lipid use during prolonged cycling in endurance-trained males as assessed by three different methodologies Am J Physiol Endocrinol Metab, June 1, 2007; 292(6): E1715 - E1723. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Prats, M. Donsmark, K. Qvortrup, C. Londos, C. Sztalryd, C. Holm, H. Galbo, and T. Ploug Decrease in intramuscular lipid droplets and translocation of HSL in response to muscle contraction and epinephrine J. Lipid Res., November 1, 2006; 47(11): 2392 - 2399. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Roepstorff, M. Donsmark, M. Thiele, B. Vistisen, G. Stewart, K. Vissing, P. Schjerling, D. G. Hardie, H. Galbo, and B. Kiens Sex differences in hormone-sensitive lipase expression, activity, and phosphorylation in skeletal muscle at rest and during exercise Am J Physiol Endocrinol Metab, November 1, 2006; 291(5): E1106 - E1114. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. Kiens Skeletal Muscle Lipid Metabolism in Exercise and Insulin Resistance Physiol Rev, January 1, 2006; 86(1): 205 - 243. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. J. C. van Loon Use of intramuscular triacylglycerol as a substrate source during exercise in humans J Appl Physiol, October 1, 2004; 97(4): 1170 - 1187. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. J. Watt, A. G. Holmes, G. R. Steinberg, J. L. Mesa, B. E. Kemp, and M. A. Febbraio Reduced plasma FFA availability increases net triacylglycerol degradation, but not GPAT or HSL activity, in human skeletal muscle Am J Physiol Endocrinol Metab, July 1, 2004; 287(1): E120 - E127. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Z. Guo, M. J. Watt, G. G. F. Heigenhauser, and L. L. Spriet Muscle fat utilization during exercise: controversial only methodologically J Appl Physiol, April 1, 2004; 96(4): 1569 - 1570. [Full Text] [PDF]
|
|
|
|
 |
|
 S. R. Stannard and N. A. Johnson Insulin resistance and elevated triglyceride in muscle: more important for survival than 'thrifty' genes? J. Physiol., February 1, 2004; 554(3): 595 - 607. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. J. Watt, P. Krustrup, N. H. Secher, B. Saltin, B. K. Pedersen, and M. A. Febbraio Glucose ingestion blunts hormone-sensitive lipase activity in contracting human skeletal muscle Am J Physiol Endocrinol Metab, January 1, 2004; 286(1): E144 - E150. [Abstract] [Full Text]
|
|
|
|
|
|
L. J. C. van Loon, V. B. Schrauwen-Hinderling, R. Koopman, A. J. M. Wagenmakers, M. K. C. Hesselink, G. Schaart, M. E. Kooi, and W. H. M. Saris Influence of prolonged endurance cycling and recovery diet on intramuscular triglyceride content in trained males Am J Physiol Endocrinol Metab, October 1, 2003; 285(4): E804 - E811. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. K. Gan, A. D. Kriketos, B. A. Ellis, C. H. Thompson, E. W. Kraegen, and D. J. Chisholm Changes in Aerobic Capacity and Visceral Fat but not Myocyte Lipid Levels Predict Increased Insulin Action After Exercise in Overweight and Obese Men Diabetes Care, June 1, 2003; 26(6): 1706 - 1713. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Hagstrom-Toft, V. Qvisth, I. Nennesmo, M. Ryden, H. Bolinder, S. Enoksson, J. Bolinder, and P. Arner Marked Heterogeneity of Human Skeletal Muscle Lipolysis at Rest Diabetes, December 1, 2002; 51(12): 3376 - 3383. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. R. Stannard, M. W. Thompson, K. Fairbairn, B. Huard, T. Sachinwalla, and C. H. Thompson Fasting for 72 h increases intramyocellular lipid content in nondiabetic, physically fit men Am J Physiol Endocrinol Metab, December 1, 2002; 283(6): E1185 - E1191. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. J. Watt, G. J. F. Heigenhauser, and L. L. Spriet Intramuscular triacylglycerol utilization in human skeletal muscle during exercise: is there a controversy? J Appl Physiol, October 1, 2002; 93(4): 1185 - 1195. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. A. Houmard, C. J. Tanner, C. Yu, P. G. Cunningham, W. J. Pories, K. G. MacDonald, and G. I. Shulman Effect of Weight Loss on Insulin Sensitivity and Intramuscular Long-Chain Fatty Acyl-CoAs in Morbidly Obese Subjects Diabetes, October 1, 2002; 51(10): 2959 - 2963. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Howald, C. Boesch, R. Kreis, S. Matter, R. Billeter, B. Essen-Gustavsson, and H. Hoppeler Content of intramyocellular lipids derived by electron microscopy, biochemical assays, and 1H-MR spectroscopy J Appl Physiol, June 1, 2002; 92(6): 2264 - 2272. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. E. Larson-Meyer, B. R. Newcomer, and G. R. Hunter Influence of endurance running and recovery diet on intramyocellular lipid content in women: a 1H NMR study Am J Physiol Endocrinol Metab, January 1, 2002; 282(1): E95 - E106. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. H. Goodpaster, J. He, S. Watkins, and D. E. Kelley Skeletal Muscle Lipid Content and Insulin Resistance: Evidence for a Paradox in Endurance-Trained Athletes J. Clin. Endocrinol. Metab., December 1, 2001; 86(12): 5755 - 5761. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Decombaz, B. Schmitt, M. Ith, B. Decarli, P. Diem, R. Kreis, H. Hoppeler, and C. Boesch Postexercise fat intake repletes intramyocellular lipids but no faster in trained than in sedentary subjects Am J Physiol Regulatory Integrative Comp Physiol, September 1, 2001; 281(3): R760 - R769. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J.-H. Hwang, J. W. Pan, S. Heydari, H. P. Hetherington, and D. T. Stein Regional differences in intramyocellular lipids in humans observed by in vivo 1H-MR spectroscopic imaging J Appl Physiol, April 1, 2001; 90(4): 1267 - 1274. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Mingrone, A. Bertuzzi, E. Capristo, A. V. Greco, M. Manco, A. Pietrobelli, S. Salinari, and S. B. Heymsfield Unreliable use of standard muscle hydration value in obesity Am J Physiol Endocrinol Metab, February 1, 2001; 280(2): E365 - E371. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. F. Horowitz and S. Klein Oxidation of nonplasma fatty acids during exercise is increased in women with abdominal obesity J Appl Physiol, December 1, 2000; 89(6): 2276 - 2282. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Z. Guo, B. Burguera, and M. D. Jensen Kinetics of intramuscular triglyceride fatty acids in exercising humans J Appl Physiol, November 1, 2000; 89(5): 2057 - 2064. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. A. Ellis, A. Poynten, A. J. Lowy, S. M. Furler, D. J. Chisholm, E. W. Kraegen, and G. J. Cooney Long-chain acyl-CoA esters as indicators of lipid metabolism and insulin sensitivity in rat and human muscle Am J Physiol Endocrinol Metab, September 1, 2000; 279(3): E554 - E560. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. F Horowitz and S. Klein Lipid metabolism during endurance exercise Am. J. Clinical Nutrition, August 1, 2000; 72(2): 558S - 563. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. H. Goodpaster, D. E. Kelley, F. L. Thaete, J. He, and R. Ross Skeletal muscle attenuation determined by computed tomography is associated with skeletal muscle lipid content J Appl Physiol, July 1, 2000; 89(1): 104 - 110. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Rico-Sanz, E. L. Thomas, G. Jenkinson, S. Mierisova, R. Iles, and J. D. Bell Diversity in levels of intracellular total creatine and triglycerides in human skeletal muscles observed by 1H-MRS J Appl Physiol, December 1, 1999; 87(6): 2068 - 2072. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. S. Szczepaniak, E. E. Babcock, F. Schick, R. L. Dobbins, A. Garg, D. K. Burns, J. D. McGarry, and D. T. Stein Measurement of intracellular triglyceride stores by H spectroscopy: validation in vivo Am J Physiol Endocrinol Metab, May 1, 1999; 276(5): E977 - E989. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. C. Bergman, G. E. Butterfield, E. E. Wolfel, G. A. Casazza, G. D. Lopaschuk, and G. A. Brooks Evaluation of exercise and training on muscle lipid metabolism Am J Physiol Endocrinol Metab, January 1, 1999; 276(1): E106 - E117. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. J. Dyck and A. Bonen Muscle contraction increases palmitate esterification and oxidation and triacylglycerol oxidation Am J Physiol Endocrinol Metab, November 1, 1998; 275(5): E888 - E896. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Kiens and E. A. Richter Utilization of skeletal muscle triacylglycerol during postexercise recovery in humans Am J Physiol Endocrinol Metab, August 1, 1998; 275(2): E332 - E337. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Chesley, R. A. Howlett, G. J. F. Heigenhauser, E. Hultman, and L. L. Spriet Regulation of muscle glycogenolytic flux during intense aerobic exercise after caffeine ingestion Am J Physiol Regulatory Integrative Comp Physiol, August 1, 1998; 275(2): R596 - R603. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. H. Steffensen, C. Roepstorff, M. Madsen, and B. Kiens Myocellular triacylglycerol breakdown in females but not in males during exercise Am J Physiol Endocrinol Metab, March 1, 2002; 282(3): E634 - E642. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Visit Other APS Journals Online |
