| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Journal of Applied Physiology, Vol 81, Issue 1 123-132, Copyright © 1996 by American Physiological Society
ARTICLES |
V. J. Caiozzo, F. Haddad, M. J. Baker, R. E. Herrick, N. Prietto and K. M. Baldwin
Department of Physiology, College of Medicine, University of California, Irvine 92717, USA.
This study examined the effects of microgravity (14 days) on 1) the contractile properties of the soleus (Sol), an antigravity skeletal muscle; and 2) the myosin heavy chain (MHC) protein and mRNA isoform content of the Sol, vastus intermedius (VI), plantaris (Plan), and tibialis anterior (TA) muscles. The force-velocity relationships of the flight Sol muscles had a significant reduction in maximal isometric tension (-37%) and a corresponding increase in maximal shortening velocity (+20%). Additionally, the force-frequency relationship of the flight Sol muscles was shifted to the right of the ground-based control group. Microgravity had the greatest effect on muscle fiber composition in the Sol muscle, with a reduction in slow muscle fibers and a corresponding increase in muscle fibers categorized as hybrid fibers. The estimated absolute MHC isoform content was altered to the greatest extent in the Sol and VI muscles, with significant decreases and elevations in the slow type I and fast type IIX MHC protein isoforms, respectively. Consistent with the protein data, both the flight Sol and VI muscles exhibited significant elevations in the fast type IIX MHC mRNA isoform. In contrast, however, the flight Plan and TA groups had significant increases in the fast type IIB MHC mRNA isoform content without corresponding changes at the protein level. The results of this study suggest that spaceflight of even short duration produces important changes in the contractile properties of antigravity skeletal muscle. These changes are mediated by alterations in MHC phenotype and reductions in muscle mass. In some instances, the alterations in MHC mRNA isoform content seemed to be uncoupled from those occurring at the protein level. This apparent uncoupling between mRNA and protein expression demonstrates that the effects of microgravity must be better understood at the transcriptional, translational, and post-translational levels.
This article has been cited by other articles:
|
|
 |
|
  H. Shi, C. Zeng, A. Ricome, K. M. Hannon, A. L. Grant, and D. E. Gerrard Extracellular signal-regulated kinase pathway is differentially involved in beta-agonist-induced hypertrophy in slow and fast muscles Am J Physiol Cell Physiol, May 1, 2007; 292(5): C1681 - C1689. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. L. Nichols, N. Zhang, and X. Wen Proteomics and genomics of microgravity Physiol Genomics, September 14, 2006; 26(3): 163 - 171. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Higashibata, N. J. Szewczyk, C. A. Conley, M. Imamizo-Sato, A. Higashitani, and N. Ishioka Decreased expression of myogenic transcription factors and myosin heavy chains in Caenorhabditis elegans muscles developed during spaceflight J. Exp. Biol., August 15, 2006; 209(16): 3209 - 3218. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Seo, K. Lee, K. Park, K. Bae, and I. Choi A Proteomic Assessment of Muscle Contractile Alterations during Unloading and Reloading J. Biochem., January 1, 2006; 139(1): 71 - 80. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W. Gao, P. J. Reiser, C. C. Coss, M. A. Phelps, J. D. Kearbey, D. D. Miller, and J. T. Dalton Selective Androgen Receptor Modulator Treatment Improves Muscle Strength and Body Composition and Prevents Bone Loss in Orchidectomized Rats Endocrinology, November 1, 2005; 146(11): 4887 - 4897. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. P. Stein and C. E. Wade Metabolic Consequences of Muscle Disuse Atrophy J. Nutr., July 1, 2005; 135(7): 1824S - 1828S. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. P Mattson and J. C Martin Emphysema-induced reductions in locomotory skeletal muscle contractile function Exp Physiol, July 1, 2005; 90(4): 519 - 525. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. Haddad, K. M. Baldwin, and P. A. Tesch Pretranslational markers of contractile protein expression in human skeletal muscle: effect of limb unloading plus resistance exercise J Appl Physiol, January 1, 2005; 98(1): 46 - 52. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Gallo, T. Gordon, N. Tyreman, Y. Shu, and C. T. Putman Reliability of isolated isometric function measures in rat muscles composed of different fibre types Exp Physiol, September 1, 2004; 89(5): 583 - 592. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Trappe, T. Trappe, P. Gallagher, M. Harber, B. Alkner, and P. Tesch Human single muscle fibre function with 84 day bed-rest and resistance exercise J. Physiol., June 1, 2004; 557(2): 501 - 513. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. R. Adams, V. J. Caiozzo, and K. M. Baldwin Skeletal muscle unweighting: spaceflight and ground-based models J Appl Physiol, December 1, 2003; 95(6): 2185 - 2201. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. C. Harrison, D. L. Allen, B. Girten, L. S. Stodieck, P. J. Kostenuik, T. A. Bateman, S. Morony, D. Lacey, and L. A. Leinwand Skeletal muscle adaptations to microgravity exposure in the mouse J Appl Physiol, December 1, 2003; 95(6): 2462 - 2470. [Abstract] [Full Text]
|
|
|
|
|
|
G. M. Diffee, K. Kalfas, S. Al-Majid, and D. O. McCarthy Altered expression of skeletal muscle myosin isoforms in cancer cachexia Am J Physiol Cell Physiol, November 1, 2002; 283(5): C1376 - C1382. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Yang, J. Luo, J. Bourdon, M.-C. Lin, S. B. Gottfried, and B. J. Petrof Controlled Mechanical Ventilation Leads to Remodeling of the Rat Diaphragm Am. J. Respir. Crit. Care Med., October 15, 2002; 166(8): 1135 - 1140. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. J. Talmadge, R. R. Roy, V. J. Caiozzo, and V. R. Edgerton Mechanical properties of rat soleus after long-term spinal cord transection J Appl Physiol, October 1, 2002; 93(4): 1487 - 1497. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. H. Fitts, D. R. Riley, and J. J. Widrick Functional and structural adaptations of skeletal muscle to microgravity J. Exp. Biol., March 11, 2002; 204(18): 3201 - 3208. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Yamashita-Goto, R. Okuyama, M. Honda, K. Kawasaki, K. Fujita, T. Yamada, I. Nonaka, Y. Ohira, and T. Yoshioka Maximal and submaximal forces of slow fibers in human soleus after bed rest J Appl Physiol, July 1, 2001; 91(1): 417 - 424. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Pette Plasticity in Skeletal, Cardiac, and Smooth Muscle: Historical Perspectives: Plasticity of mammalian skeletal muscle J Appl Physiol, March 1, 2001; 90(3): 1119 - 1124. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. M. Baldwin and F. Haddad Plasticity in Skeletal, Cardiac, and Smooth Muscle: Invited Review: Effects of different activity and inactivity paradigms on myosin heavy chain gene expression in striated muscle J Appl Physiol, January 1, 2001; 90(1): 345 - 357. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. H. Fitts, D. R. Riley, and J. J. Widrick Physiology of a Microgravity Environment Invited Review: Microgravity and skeletal muscle J Appl Physiol, August 1, 2000; 89(2): 823 - 839. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. F Coyle Physical activity as a metabolic stressor Am. J. Clinical Nutrition, August 1, 2000; 72(2): 512S - 520. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. R. Adams, S. A. McCue, P. W. Bodell, M. Zeng, and K. M. Baldwin Effects of spaceflight and thyroid deficiency on hindlimb development. I. Muscle mass and IGF-I expression J Appl Physiol, March 1, 2000; 88(3): 894 - 903. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. R. Adams, F. Haddad, S. A. McCue, P. W. Bodell, M. Zeng, L. Qin, A. X. Qin, and K. M. Baldwin Effects of spaceflight and thyroid deficiency on rat hindlimb development. II. Expression of MHC isoforms J Appl Physiol, March 1, 2000; 88(3): 904 - 916. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. P. Grichko, A. Heywood-Cooksey, K. R. Kidd, and R. H. Fitts Substrate profile in rat soleus muscle fibers after hindlimb unloading and fatigue J Appl Physiol, February 1, 2000; 88(2): 473 - 478. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Cormery, F. Pons, J.-F. Marini, and P. F. Gardiner Myosin heavy chains in fibers of TTX-paralyzed rat soleus and medial gastrocnemius muscles J Appl Physiol, January 1, 2000; 88(1): 66 - 76. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Stevens, K. R. Sultan, H. Peuker, B. Gohlsch, Y. Mounier, and D. Pette Time-dependent changes in myosin heavy chain mRNA and protein isoforms in unloaded soleus muscle of rat Am J Physiol Cell Physiol, December 1, 1999; 277(6): C1044 - C1049. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Cros, J. Muller, S. Bouju, G. Pietu, C. Jacquet, J. J. Leger, J.-F. Marini, and C. A. Dechesne Upregulation of M-creatine kinase and glyceraldehyde3-phosphate dehydrogenase: two markers of muscle disuse Am J Physiol Regulatory Integrative Comp Physiol, February 1, 1999; 276(2): R308 - R316. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. H. Lecker, V. Solomon, W. E. Mitch, and A. L. Goldberg Muscle Protein Breakdown and the Critical Role of the Ubiquitin-Proteasome Pathway in Normal and Disease States J. Nutr., January 1, 1999; 129(1): 227S - 227S. [Full Text] [PDF]
|
|
|
|
|
|
F. Haddad, A. X. Qin, M. Zeng, S. A. McCue, and K. M. Baldwin Interaction of hyperthyroidism and hindlimb suspension on skeletal myosin heavy chain expression J Appl Physiol, December 1, 1998; 85(6): 2227 - 2236. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. J. Widrick, K. M. Norenberg, J. G. Romatowski, C. A. Blaser, M. Karhanek, J. Sherwood, S. W. Trappe, T. A. Trappe, D. L. Costill, and R. H. Fitts Force-velocity-power and force-pCa relationships of human soleus fibers after 17 days of bed rest J Appl Physiol, November 1, 1998; 85(5): 1949 - 1956. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. E. Dupont-Versteegden, J. D. Houle, C. M. Gurley, and C. A. Peterson Early changes in muscle fiber size and gene expression in response to spinal cord transection and exercise Am J Physiol Cell Physiol, October 1, 1998; 275(4): C1124 - C1133. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Vijayan, J. L. Thompson, and D. A. Riley Sarcomere lesion damage occurs mainly in slow fibers of reloaded rat adductor longus muscles J Appl Physiol, September 1, 1998; 85(3): 1017 - 1023. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Yang, J. Bourdon, S. B. Gottfried, W. A. Zin, and B. J. Petrof Regulation of myosin heavy chain gene expression after short-term diaphragm inactivation Am J Physiol Lung Cell Mol Physiol, June 1, 1998; 274(6): L980 - L989. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Wright, F. Haddad, A. X. Qin, and K. M. Baldwin Analysis of myosin heavy chain mRNA expression by RT-PCR J Appl Physiol, October 1, 1997; 83(4): 1389 - 1396. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Inobe, I. Inobe, G. R. Adams, K. M. Baldwin, and S.'I. Takeda Effects of microgravity on myogenic factor expressions during postnatal development of rat skeletal muscle J Appl Physiol, May 1, 2002; 92(5): 1936 - 1942. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Visit Other APS Journals Online |
