| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Journal of Applied Physiology, Vol 80, Issue 6 2120-2133, Copyright © 1996 by American Physiological Society
ARTICLES |
S. M. Johnson, J. C. Smith and J. L. Feldman
Department of Physiological Science, University of California at Los Angeles 90095-1527, USA.
Slice preparations from neonatal rat medulla that generate respiratory rhythm in vitro were used to test for Gi/o protein-mediated mechanisms affecting breathing rhythm in mammals. The frequency of inspiratory motor discharge recorded from hypoglossal (XII) nerve roots decreased with bath application of gamma-aminobutyric acid (GABA) and norepinephrine, as well as agonists specific for GABAB, alpha 2-adrenergic, and mu-opioid receptors; 5-hydroxytryptamine had little effect on frequency. Microinjection of these specific agonists into the pre-Botzinger complex, the site of respiratory rhythm generation in vitro, also decreased frequency. In contrast, substance P (SP) increased frequency when it was bath applied or microinjected into the pre-Botzinger complex. To test for involvement of Gi/o proteins, pertussis toxin (PTX) was injected into the cerebrospinal fluid of newborn rats, and slices from these animals were tested 48 h later for block of drug effects on rhythm. In PTX-treated slices the frequency decrease due to GABAB, mu-opioid, and alpha 2-adrenergic receptor activation was attenuated (P < or = 0.05), whereas the SP receptor-mediated response was unaltered. To test for involvement of K+ conductances linked to Gi/o proteins Ba2+ (0.2 mM) was added to the bath before application of drugs. Ba2+ attenuated the decrease in frequency associated with GABAB (P < or = 0.05) and mu-opioid (0.10 < or = P < or = 0.05) receptor activation, whereas the alpha 2-adrenergic and SP responses were unaltered. We conclude that GABAB and mu-opioid, but not alpha 2-adrenergic and SP, receptor activation modulates respiratory frequency via a Gi/o protein-dependent Ba(2+)-sensitive ionic conductance mechanism on neurons within the medullary locus for rhythm generation. This mechanism may be a convergent pathway for control of respiratory frequency.
This article has been cited by other articles:
|
|
 |
|
  J. A. Hayes, J. L. Mendenhall, B. R. Brush, and C. A. Del Negro 4-Aminopyridine-sensitive outward currents in preBotzinger complex neurons influence respiratory rhythm generation in neonatal mice J. Physiol., April 1, 2008; 586(7): 1921 - 1936. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Koizumi and J. C. Smith Persistent Na+ and K+-Dominated Leak Currents Contribute to Respiratory Rhythm Generation in the Pre-Botzinger Complex In Vitro J. Neurosci., February 13, 2008; 28(7): 1773 - 1785. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Y. Fong and J. T. Potts Neurokinin-1 Receptors Modulate the Excitability of Expiratory Neurons in the Ventral Respiratory Group J Neurophysiol, February 1, 2008; 99(2): 900 - 914. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Ruangkittisakul, L. Secchia, T. D. Bornes, D. M. Palathinkal, and K. Ballanyi Dependence on extracellular Ca2+/K+ antagonism of inspiratory centre rhythms in slices and en bloc preparations of newborn rat brainstem J. Physiol., October 15, 2007; 584(2): 489 - 508. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. K. Purvis, J. C. Smith, H. Koizumi, and R. J. Butera Intrinsic Bursters Increase the Robustness of Rhythm Generation in an Excitatory Network J Neurophysiol, February 1, 2007; 97(2): 1515 - 1526. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. R. Lorier, A. G. Huxtable, D. M. Robinson, J. Lipski, G. D. Housley, and G. D. Funk P2Y1 Receptor Modulation of the Pre-Botzinger Complex Inspiratory Rhythm Generating Network In Vitro J. Neurosci., January 31, 2007; 27(5): 993 - 1005. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Y. Fong and J. T. Potts Neurokinin-1 receptor activation in Botzinger complex evokes bradypnoea J. Physiol., September 15, 2006; 575(3): 869 - 885. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Ren and J. J. Greer Neurosteroid modulation of respiratory rhythm in rats during the perinatal period J. Physiol., July 15, 2006; 574(2): 535 - 546. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Ren and J. J. Greer Modulation of respiratory rhythmogenesis by chloride-mediated conductances during the perinatal period. J. Neurosci., April 5, 2006; 26(14): 3721 - 3730. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. J. Greer, G. D. Funk, and K. Ballanyi Preparing for the first breath: prenatal maturation of respiratory neural control J. Physiol., February 1, 2006; 570(3): 437 - 444. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Choi, W.-L. Liao, K. M. Newton, R. C. Onario, A. M. King, F. C. Desilets, E. J. Woodard, M. E. Eichler, W. R. Frontera, S. Sabharwal, et al. Respiratory Abnormalities Resulting from Midcervical Spinal Cord Injury and their Reversal by Serotonin 1A Agonists in Conscious Rats J. Neurosci., May 4, 2005; 25(18): 4550 - 4559. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Adachi, D. M. Robinson, G. B. Miles, and G. D. Funk Noradrenergic modulation of XII motoneuron inspiratory activity does not involve {alpha}2-receptor inhibition of the Ih current or presynaptic glutamate release J Appl Physiol, April 1, 2005; 98(4): 1297 - 1308. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Pagliardini, T. Adachi, J. Ren, G. D. Funk, and J. J. Greer Fluorescent Tagging of Rhythmically Active Respiratory Neurons within the Pre-Botzinger Complex of Rat Medullary Slice Preparations J. Neurosci., March 9, 2005; 25(10): 2591 - 2596. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. M. Lalley {micro}-Opioid receptor agonist effects on medullary respiratory neurons in the cat: evidence for involvement in certain types of ventilatory disturbances Am J Physiol Regulatory Integrative Comp Physiol, December 1, 2003; 285(6): R1287 - R1304. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. D. Teng, M. Bingaman, A. M. Taveira-DaSilva, P. P. Pace, R. A. Gillis, and J. R. Wrathall Serotonin 1A Receptor Agonists Reverse Respiratory Abnormalities in Spinal Cord-Injured Rats J. Neurosci., May 15, 2003; 23(10): 4182 - 4189. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Mao, L. Li, M. McManus, J. Wu, N. Cui, and C. Jiang Molecular Determinants for Activation of G-protein-coupled Inward Rectifier K+ (GIRK) Channels by Extracellular Acidosis J. Biol. Chem., November 22, 2002; 277(48): 46166 - 46171. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. Fenik, V. Marchenko, P. Janssen, R. O. Davies, and L. Kubin A5 cells are silenced when REM sleep-like signs are elicited by pontine carbachol J Appl Physiol, October 1, 2002; 93(4): 1448 - 1456. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Telgkamp, Y. Q. Cao, A. I. Basbaum, and J.-M. Ramirez Long-Term Deprivation of Substance P in PPT-A Mutant Mice Alters the Anoxic Response of the Isolated Respiratory Network J Neurophysiol, July 1, 2002; 88(1): 206 - 213. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. G. Guyenet, C. P. Sevigny, M. C. Weston, and R. L. Stornetta Neurokinin-1 Receptor-Expressing Cells of the Ventral Respiratory Group Are Functionally Heterogeneous and Predominantly Glutamatergic J. Neurosci., May 1, 2002; 22(9): 3806 - 3816. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. M. Johnson, J. E. R. Wilkerson, D. R. Henderson, M. R. Wenninger, and G. S. Mitchell Serotonin elicits long-lasting enhancement of rhythmic respiratory activity in turtle brain stems in vitro J Appl Physiol, December 1, 2001; 91(6): 2703 - 2712. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. A. Del Negro, S. M. Johnson, R. J. Butera, and J. C. Smith Models of Respiratory Rhythm Generation in the Pre-Botzinger Complex. III. Experimental Tests of Model Predictions J Neurophysiol, July 1, 2001; 86(1): 59 - 74. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. A. Gray, J. C. Rekling, C. M. Bocchiaro, and J. L. Feldman Modulation of Respiratory Frequency by Peptidergic Input to Rhythmogenic Neurons in the PreBötzinger Complex Science, November 19, 1999; 286(5444): 1566 - 1568. [Abstract] [Full Text]
|
|
|
|
|
|
S. L. Mironov, K. Langohr, and D. W. Richter A1 Adenosine Receptors Modulate Respiratory Activity of the Neonatal Mouse Via the cAMP-Mediated Signaling Pathway J Neurophysiol, January 1, 1999; 81(1): 247 - 255. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. A. Waters, A. Laferriere, J. Paquette, C. Goodyer, and I. R. Moss Curtailed respiration by repeated vs. isolated hypoxia in maturing piglets is unrelated to NTS ME or SP levels J Appl Physiol, August 1, 1997; 83(2): 522 - 529. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. M. Lalley, O. Pierrefiche, A. M. Bischoff, and D. W. Richter cAMP-Dependent Protein Kinase Modulates Expiratory Neurons In Vivo J Neurophysiol, March 1, 1997; 77(3): 1119 - 1131. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Visit Other APS Journals Online |
