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J Appl Physiol 80: 1258-1262, 1996;
8750-7587/96 $5.00
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Journal of Applied Physiology, Vol 80, Issue 4 1258-1262, Copyright © 1996 by American Physiological Society

ARTICLES

A modified rebreathing technique using an infrared gas analyzer

K. W. Barazanji, M. Ramanathan, R. L. Johnson Jr and C. C. Hsia
Department of Internal Medicine, University of Texas Southwestern Medical Center, Dallas 75235-9034, USA.

To lower the cost and improve accessibility of the rebreathing technique for measuring cardiopulmonary function during exercise, we implemented a fast-response infrared (IR) gas-analyzer system to simultaneously measure lung diffusing capacity, cardiac output lung tissue volume, and lung volume by a rebreathing technique in five healthy subjects at rest and during steady-state exercise. Interferences by water vapor and CO2 on the analyzer were determined and corrected for. During rebreathing, a gas mixture of 0.4% C2H2-0.3% CH4-9% He-30% O2, and either 0.3% C18O or 0.3% C16O in a balance of N2 was simultaneously sampled by both a mass spectrometer and the IR analyzer, permitting paired comparisons. Measurements obtained by the two devices were not significantly different. We conclude that this modified rebreathing technique using the IR analyzer is accurate for the measurement of cardiopulmonary function at rest and during exercise.


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