Journal of Applied Physiology Fuel your research with LabChart
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

J Appl Physiol 79: 1615-1628, 1995;
8750-7587/95 $5.00
This Article
Right arrow Full Text (PDF)
Right arrow Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Bastacky, J.
Right arrow Articles by Clements, J. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Bastacky, J.
Right arrow Articles by Clements, J. A.

Journal of Applied Physiology, Vol 79, Issue 5 1615-1628, Copyright © 1995 by American Physiological Society

ARTICLES

Alveolar lining layer is thin and continuous: low-temperature scanning electron microscopy of rat lung

J. Bastacky, C. Y. Lee, J. Goerke, H. Koushafar, D. Yager, L. Kenaga, T. P. Speed, Y. Chen and J. A. Clements
Life Sciences Division, Lawrence Berkeley Laboratory, University of California, Berkeley 94720 USA. sjbastacky@lbl.gov

The low-temperature electron microscope, which preserves aqueous structures as solid water at liquid nitrogen temperature, was used to image the alveolar lining layer, including surfactant and its aqueous subphase, of air-filled lungs frozen in anesthetized rats at 15-cmH2O transpulmonary pressure. Lining layer thickness was measured on cross fractures of walls of the outermost subpleural alveoli that could be solidified with metal mirror cryofixation at rates sufficient to limit ice crystal growth to 10 nm and prevent appreciable water movement. The thickness of the liquid layer averaged 0.14 micron over relatively flat portions of the alveolar walls, 0.89 micron at the alveolar wall junctions, and 0.09 micron over the protruding features (9 rats, 20 walls, 16 junctions, and 146 areas), for an area-weighted average thickness of 0.2 micron. The alveolar lining layer appears continuous, submerging epithelial cell microvilli and intercellular junctional ridges; varies from a few nanometers to several micrometers in thickness, and serves to smooth the alveolar air-liquid interface in lungs inflated to zone 1 or 2 conditions.


This article has been cited by other articles:


Home page
 J. Appl. Physiol.Home page
C. E. Perlman and J. Bhattacharya
Alveolar expansion imaged by optical sectioning microscopy
J Appl Physiol, September 1, 2007; 103(3): 1037 - 1044.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
J. Lindert, C. E. Perlman, K. Parthasarathi, and J. Bhattacharya
Chloride-Dependent Secretion of Alveolar Wall Liquid Determined by Optical-Sectioning Microscopy
Am. J. Respir. Cell Mol. Biol., June 1, 2007; 36(6): 688 - 696.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
M. D. Johnson, H.-F. Bao, M. N. Helms, X.-J. Chen, Z. Tigue, L. Jain, L. G. Dobbs, and D. C. Eaton
Functional ion channels in pulmonary alveolar type I cells support a role for type I cells in lung ion transport
PNAS, March 28, 2006; 103(13): 4964 - 4969.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
M. Geiser and S. Bastian
Surface-lining layer of airways in cystic fibrosis mice
Am J Physiol Lung Cell Mol Physiol, December 1, 2003; 285(6): L1277 - L1285.
[Abstract] [Full Text] [PDF]

Home page
 Biophys. JHome page
W. R. Schief, M. Antia, B. M. Discher, S. B. Hall, and V. Vogel
Liquid-Crystalline Collapse of Pulmonary Surfactant Monolayers
Biophys. J., June 1, 2003; 84(6): 3792 - 3806.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
C. G. De Pasquale, A. D. Bersten, I. R. Doyle, P. E. Aylward, and L. F. Arnolda
Infarct-induced chronic heart failure increases bidirectional protein movement across the alveolocapillary barrier
Am J Physiol Heart Circ Physiol, June 1, 2003; 284(6): H2136 - H2145.
[Abstract] [Full Text] [PDF]

Home page
 J. Lipid Res.Home page
S.-H. Yu and F. Possmayer
Lipid compositional analysis of pulmonary surfactant monolayers and monolayer-associated reservoirs
J. Lipid Res., March 1, 2003; 44(3): 621 - 629.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
H. Bachofen, U. Gerber, and S. Schurch
Effects of fixatives on function of pulmonary surfactant
J Appl Physiol, September 1, 2002; 93(3): 911 - 916.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
G. P. Topulos, R. E. Brown, and J. P. Butler
Increased surface tension decreases pulmonary capillary volume and compliance
J Appl Physiol, September 1, 2002; 93(3): 1023 - 1029.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
K. G. Davidson, A. D. Bersten, H. A. Barr, K. D. Dowling, T. E. Nicholas, and I. R. Doyle
Endotoxin Induces Respiratory Failure and Increases Surfactant Turnover and Respiration Independent of Alveolocapillary Injury in Rats
Am. J. Respir. Crit. Care Med., June 1, 2002; 165(11): 1516 - 1525.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S. Murakami, D. Iwaki, H. Mitsuzawa, H. Sano, H. Takahashi, D. R. Voelker, T. Akino, and Y. Kuroki
Surfactant Protein A Inhibits Peptidoglycan-induced Tumor Necrosis Factor-alpha Secretion in U937 Cells and Alveolar Macrophages by Direct Interaction with Toll-like Receptor 2
J. Biol. Chem., February 22, 2002; 277(9): 6830 - 6837.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
L. M. Connor, A. Bidani, J. Goerke, J. A. Clements, and E. M. Postlethwait
NO2 interfacial transfer is reduced by phospholipid monolayers
J Appl Physiol, November 1, 2001; 91(5): 2024 - 2034.
[Abstract] [Full Text] [PDF]

Home page
 PhysiologyHome page
P. Dietl, T. Haller, N. Mair, and M. Frick
Mechanisms of Surfactant Exocytosis in Alveolar Type II Cells In Vitro and In Vivo
Physiology, October 1, 2001; 16(5): 239 - 243.
[Abstract] [Full Text] [PDF]

Home page
 J. Lipid Res.Home page
S.-H. Yu and F. Possmayer
Dipalmitoylphosphatidylcholine and cholesterol in monolayers spread from adsorbed films of pulmonary surfactant
J. Lipid Res., September 1, 2001; 42(9): 1421 - 1429.
[Abstract] [Full Text] [PDF]

Home page
 Br J AnaesthHome page
K.L. Dorrington and J.D. Young
Editorial III: Development of the concept of a liquid pulmonary alveolar lining layer
Br. J. Anaesth., May 1, 2001; 86(5): 614 - 617.
[Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
W. T. Watford, A. J. Ghio, and J. R. Wright
Complement-mediated host defense in the lung
Am J Physiol Lung Cell Mol Physiol, November 1, 2000; 279(5): L790 - L798.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
E. M. Scarpelli and B. A. Hills
Opposing views on the alveolar surface, alveolar models, and the role of surfactant
J Appl Physiol, August 1, 2000; 89(2): 408 - 412.
[Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
A. FEHRENBACH, M. OCHS, T. WARNECKE, T. WAHLERS, T. WITTWER, A. SCHMIEDL, S. ELKI, D. MEYER, J. RICHTER, and H. FEHRENBACH
Beneficial Effect of Lung Preservation Is Related to Ultrastructural Integrity of Tubular Myelin after Experimental Ischemia and Reperfusion
Am. J. Respir. Crit. Care Med., June 1, 2000; 161(6): 2058 - 2065.
[Abstract] [Full Text]

Home page
 Am. J. Respir. Crit. Care Med.Home page
M. KAWASHIMA, T. BANDO, T. NAKAMURA, N. ISOWA, M. LIU, S. TOYOKUNI, S. HITOMI, and H. WADA
Cytoprotective Effects of Nitroglycerin in Ischemia-Reperfusion-Induced Lung Injury
Am. J. Respir. Crit. Care Med., March 1, 2000; 161(3): 935 - 943.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
T. E. DeCoursey
Hypothesis: do voltage-gated H+ channels in alveolar epithelial cells contribute to CO2 elimination by the lung?
Am J Physiol Cell Physiol, January 1, 2000; 278(1): C1 - C10.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
B. A. Hills
An alternative view of the role(s) of surfactant and the alveolar model
J Appl Physiol, November 1, 1999; 87(5): 1567 - 1583.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
J. Savov, R. Silbajoris, and S. L. Young
Mechanical ventilation of rat lung: effect on surfactant forms
Am J Physiol Lung Cell Mol Physiol, August 1, 1999; 277(2): L320 - L326.
[Abstract] [Full Text] [PDF]

Home page
 Arch. Dis. Child. Fetal Neonatal Ed.Home page
B A HILLS and I B MASTERS
"Dewatering" the lungs
Arch. Dis. Child. Fetal Neonatal Ed., January 1, 1999; 80(1): 78F - 78.
[Full Text]

Home page
 Am. J. Respir. Crit. Care Med.Home page
S. BAYAT, A. MENAOUAR, D. ANGLADE, H. ETTINGER, A. FRANCOIS-JOUBERT, G. BENCHETRIT, and F. A. GRIMBERT
In Vivo Quantitation of Epithelial Lining Fluid in Dog Lung
Am. J. Respir. Crit. Care Med., December 1, 1998; 158(6): 1715 - 1723.
[Abstract] [Full Text] [PDF]

Home page
 Arch. Dis. Child. Fetal Neonatal Ed.Home page
B. A HILLS, I B. MASTERS;, and D V WALTERS
"Dewatering" of the lungs at birth • Commentary
Arch. Dis. Child. Fetal Neonatal Ed., November 1, 1998; 79(3): 221F - 222.
[Full Text]

Home page
 J. Appl. Physiol.Home page
B. A. Hills;, C. A. Piantadosi, Y.-C. T. Huang, and S. L. Young
Letters to the Editor
J Appl Physiol, August 1, 1998; 85(2): 770 - 772.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
E. M. Postlethwait, R. Cueto, L. W. Velsor, and W. A. Pryor
O3-induced formation of bioactive lipids: estimated surface concentrations and lining layer effects
Am J Physiol Lung Cell Mol Physiol, June 1, 1998; 274(6): L1006 - L1016.
[Abstract] [Full Text] [PDF]

Home page
 J. Lipid Res.Home page
S.-H. Yu and F. Possmayer
Interaction of pulmonary surfactant protein A with dipalmitoylphosphatidylcholine and cholesterol at the air/water interface
J. Lipid Res., March 1, 1998; 39(3): 555 - 568.
[Abstract] [Full Text]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
L. W. Velsor and E. M. Postlethwait
NO2-induced generation of extracellular reactive oxygen is mediated by epithelial lining layer antioxidants
Am J Physiol Lung Cell Mol Physiol, December 1, 1997; 273(6): L1265 - L1275.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
D. Joseph, O. Tirmizi, X.-L. Zhang, E. D. Crandall, and R. L. Lubman
Alveolar Epithelial Ion and Fluid Transport: Polarity of alveolar epithelial cell acid-base permeability
Am J Physiol Lung Cell Mol Physiol, April 1, 2002; 282(4): L675 - L683.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online