Journal of Applied Physiology, Vol 79, Issue 4 1386-1390, Copyright © 1995 by American Physiological Society

ARTICLES

Measurement of airway mucosal blood flow with dimethylether: validation with microspheres

M. Scuri, V. McCaskill, A. D. Chediak, W. M. Abraham and A. Wanner
Pulmonary Division, University of Miami School of Medicine, Mount Sinai Medical Center, Miami Beach, Florida 33140, USA.

We have recently developed a noninvasive dimethylether (DME) uptake technique to estimate airway mucosal blood flow (Qaw) in humans (12). Because it was not feasible to validate the technique directly, we undertook the present study to compare Qaw as measured by DME (QDME) and by color-coded microspheres (QM) as a standard in seven anesthetized sheep prepared with a carotid and a left atrial catheter. QDME was determined by measuring DME uptake with multiple breath holds after passive inflation with a DME-helium gas mixture, simulating the technique used in humans. After the microspheres were injected into the left atrium, the sheep were killed and the tracheal segment corresponding to the dead space from which DME uptake was determined was removed, and its mucosa was stripped and processed for microsphere counts. Mean QDME was 35.6 ml.min-1.100 g-1 wet tissue (range 9.6-98.0 ml.min-1.100 g-1) and mean QM was 29.1 ml.min-1.100 g-1 (range 7.7-91.5 ml.min-1.100 g-1). There was a strong correlation between QDME and QM (r = 0.89; P = 0.01). Intravenous nitroglycerin and vasopressin caused comparable increases and/or decreases in QDME and QM (r = 0.87; P = 0.02). This suggests that the noninvasive DME uptake method measures Qaw accurately and supports its validity in human studies.

This article has been cited by other articles:

				E. S. Mendes, G. Horvath, P. Rebolledo, M. E. Monzon, S. M. Casalino-Matsuda, and A. Wanner Effect of an inhaled glucocorticoid on endothelial function in healthy smokers J Appl Physiol, July 1, 2008; 105(1): 54 - 57. [Abstract] [Full Text] [PDF]

				P. Paredi, S. Ward, D. Cramer, P. J. Barnes, and S. A. Kharitonov Normal Bronchial Blood Flow in COPD Is Unaffected by Inhaled Corticosteroids and Correlates With Exhaled Nitric Oxide Chest, April 1, 2007; 131(4): 1075 - 1081. [Abstract] [Full Text] [PDF]

				E. S. Mendes, M. A. Campos, and A. Wanner Airway Blood Flow Reactivity in Healthy Smokers and in Ex-Smokers With or Without COPD. Chest, April 1, 2006; 129(4): 893 - 898. [Abstract] [Full Text] [PDF]

				G. Horvath and A. Wanner Inhaled corticosteroids: effects on the airway vasculature in bronchial asthma Eur. Respir. J., January 1, 2006; 27(1): 172 - 187. [Abstract] [Full Text] [PDF]

				E. S. Mendes, M. A. Campos, A. Hurtado, and A. Wanner Effect of Montelukast and Fluticasone Propionate on Airway Mucosal Blood Flow in Asthma Am. J. Respir. Crit. Care Med., May 15, 2004; 169(10): 1131 - 1134. [Abstract] [Full Text] [PDF]

				J. B. Morris, P. T. Symanowicz, J. E. Olsen, R. S. Thrall, M. M. Cloutier, and A. K. Hubbard Immediate sensory nerve-mediated respiratory responses to irritants in healthy and allergic airway-diseased mice J Appl Physiol, April 1, 2003; 94(4): 1563 - 1571. [Abstract] [Full Text] [PDF]

				J. Brieva and A. Wanner Adrenergic airway vascular smooth muscle responsiveness in healthy and asthmatic subjects J Appl Physiol, February 1, 2001; 90(2): 665 - 669. [Abstract] [Full Text] [PDF]

				S. D. KUMAR, J. L. BRIEVA, I. DANTA, and A. WANNER Transient Effect of Inhaled Fluticasone on Airway Mucosal Blood Flow in Subjects with and without Asthma Am. J. Respir. Crit. Care Med., March 1, 2000; 161(3): 918 - 921. [Abstract] [Full Text] [PDF]

				J. L. BRIEVA, I. DANTA, and A. WANNER Effect of an Inhaled Glucocorticosteroid on Airway Mucosal Blood Flow in Mild Asthma Am. J. Respir. Crit. Care Med., January 1, 2000; 161(1): 293 - 296. [Abstract] [Full Text]

				J. B. Morris, J. Stanek, and G. Gianutsos Sensory nerve-mediated immediate nasal responses to inspired acrolein J Appl Physiol, November 1, 1999; 87(5): 1877 - 1886. [Abstract] [Full Text] [PDF]

				S. D. KUMAR, M. J. EMERY, N. D. ATKINS, I. DANTA, and A. WANNER Airway Mucosal Blood Flow in Bronchial Asthma Am. J. Respir. Crit. Care Med., July 1, 1998; 158(1): 153 - 156. [Abstract] [Full Text] [PDF]