Journal of Applied Physiology, Vol 79, Issue 3 748-755, Copyright © 1995 by American Physiological Society

ARTICLES

Indomethacin does not alter natural killer cell response to 2.5 h of running

D. C. Nieman, J. C. Ahle, D. A. Henson, B. J. Warren, J. Suttles, J. M. Davis, K. S. Buckley, S. Simandle, D. E. Butterworth, O. R. Fagoaga and al. et
Department of Health, Appalachian State University, Boone, North Carolina 28608, USA.

The effect of 2.5 h of treadmill running at 75.6 +/- 0.9% maximal O2 uptake (VO2max) on natural killer (NK) cell cytotoxic activity (NKCA) was investigated in 22 experienced marathon runners (VO2max 57.9 +/- 1.1 ml.kg-1.min-1, age 38.7 +/- 1.5 yr). Blood samples were taken before (0715) and immediately after exercise (1000), with three more samples taken during 6 h of recovery (1130, 1300, and 1600). Ten sedentary controls (VO2max 34.7 +/- 1.0 ml.kg-1.min-1, age 45.3 +/- 2.3 yr) sat in the laboratory during testing and had their blood sampled at the same time points. The pattern of change in NKCA over time was significantly different between groups [F(4,27) = 6.53; P = 0.001], with the runner's NKCA dropping 51-61% below preexercise levels throughout 6 h of recovery. Preincubation of blood mononuclear cells in vitro with indomethacin had no effect on the difference in pattern of change in NKCA between groups [F(4,17) = 8.59; P = 0.001] and did not attenuate the postexercise reduction in the runners. When NKCA was adjusted on a per-NK cell basis, group differences and the postexercise decline in NKCA were eliminated [F(4,80) = 0.65; P = 0.63]. Serum cortisol and plasma epinephrine in the runners were elevated relative to control subjects during recovery from exercise, but no significant correlation with changes in NK cells or NKCA was found. These data indicate that NKCA is decreased significantly during recovery from 2.5 h of running due to a numerical redistribution of NK cells.

This article has been cited by other articles:

				J Scharhag, T Meyer, H H W Gabriel, B Schlick, O Faude, W Kindermann, and R J Shephard Does prolonged cycling of moderate intensity affect immune cell function? * Commentary Br. J. Sports Med., March 1, 2005; 39(3): 171 - 177. [Abstract] [Full Text] [PDF]

				I. Yakar, R. Melamed, G. Shakhar, K. Shakhar, E. Rosenne, N. Abudarham, G. G. Page, and S. Ben-Eliyahu Prostaglandin E2 Suppresses NK Activity In Vivo and Promotes Postoperative Tumor Metastasis in Rats Ann. Surg. Oncol., May 1, 2003; 10(4): 469 - 479. [Abstract] [Full Text] [PDF]

				W. A. Braun, M. G. Flynn, D. E. Jacks, T. McLoughlin, J. Sowash, C. P. Lambert, and E. Mylona Indomethacin does not influence natural cell-mediated cytotoxic response to endurance exercise J Appl Physiol, December 1, 1999; 87(6): 2237 - 2243. [Abstract] [Full Text] [PDF]

				M. G. Flynn, M. Fahlman, W. A. Braun, C. P. Lambert, L. E. Bouillon, P. G. Brolinson, and C. W. Armstrong Effects of resistance training on selected indexes of immune function in elderly women J Appl Physiol, June 1, 1999; 86(6): 1905 - 1913. [Abstract] [Full Text] [PDF]

				S. G. Rhind, G. A. Gannon, M. Suzui, R. J. Shephard, and P. N. Shek Indomethacin inhibits circulating PGE2 and reverses postexercise suppression of natural killer cell activity Am J Physiol Regulatory Integrative Comp Physiol, May 1, 1999; 276(5): R1496 - R1505. [Abstract] [Full Text] [PDF]

				D. C. Nieman, S. L. Nehlsen-Cannarella, O. R. Fagoaga, D. A. Henson, A. Utter, J. M. Davis, F. Williams, and D. E. Butterworth Effects of mode and carbohydrate on the granulocyte and monocyte response to intensive, prolonged exercise J Appl Physiol, April 1, 1998; 84(4): 1252 - 1259. [Abstract] [Full Text] [PDF]

				D. C. Nieman Immune response to heavy exertion J Appl Physiol, May 1, 1997; 82(5): 1385 - 1394. [Abstract] [Full Text] [PDF]