Journal of Applied Physiology, Vol 79, Issue 2 648-659, Copyright © 1995 by American Physiological Society

ARTICLES

A mathematical model for measuring blood flow in skeletal muscle with the microdialysis ethanol technique

F. Wallgren, G. Amberg, R. C. Hickner, U. Ekelund, L. Jorfeldt and J. Henriksson
Department of Mechanics, Royal Institute of Technology, Stockholm, Sweden.

A theoretical analysis of the microdialysis ethanol technique in skeletal muscle is presented, and a model governing the transport of ethanol from the microdialysis probe to the capillaries in the muscle tissue is proposed. The model is derived under the assumption of a steady-state situation, and an analytical solution is found for the outflow-to-inflow ratio of ethanol in the perfusate. Theoretically calculated results are compared with experiments, and for at least one of the two probe types used good agreement is achieved in a wide range of blood flow and perfusate flow rates. The main uncertainty factor in the theoretical calculations is the diffusivity of ethanol in muscle tissue, and the value for best agreement between theory and experiments has been used. Error estimates show that for a constant relative error in the outflow-to-inflow ratio of ethanol in the perfusate, low perfusate flow rates give better predictions of the blood flow.

This article has been cited by other articles:

				J. M. B. Newman, R. M. Ross, S. M. Richards, M. G. Clark, and S. Rattigan Insulin and contraction increase nutritive blood flow in rat muscle in vivo determined by microdialysis of L-[14C]glucose J. Physiol., November 15, 2007; 585(1): 217 - 229. [Abstract] [Full Text] [PDF]

				B. E. De Galan, P. De Mol, L. Wennekes, B. J. J. Schouwenberg, and P. Smits Preserved Sensitivity to {beta}2-Adrenergic Receptor Agonists in Patients with Type 1 Diabetes Mellitus and Hypoglycemia Unawareness J. Clin. Endocrinol. Metab., August 1, 2006; 91(8): 2878 - 2881. [Abstract] [Full Text] [PDF]

				J. L Roberts, J. M. B Newman, R. Warner, S. Rattigan, and M. G Clark Axially symmetric semi-infinite domain models of microdialysis and their application to the determination of nutritive flow in rat muscle J. Physiol., February 15, 2005; 563(1): 213 - 228. [Abstract] [Full Text] [PDF]

				M. Boschmann, C. Schroeder, N. J. Christensen, J. Tank, G. Krupp, I. Biaggioni, S. Klaus, A. M. Sharma, F. C. Luft, and J. Jordan Norepinephrine Transporter Function and Autonomic Control of Metabolism J. Clin. Endocrinol. Metab., November 1, 2002; 87(11): 5130 - 5137. [Abstract] [Full Text] [PDF]

				J. M. B. Newman, C. A. Di Maria, S. Rattigan, and M. G. Clark Nutritive blood flow affects microdialysis O/I ratio for [14C]ethanol and 3H2O in perfused rat hindlimb Am J Physiol Heart Circ Physiol, December 1, 2001; 281(6): H2731 - H2737. [Abstract] [Full Text] [PDF]

				B. Stallknecht, M. Donsmark, L. H. Enevoldsen, J. D. Fluckey, and H. Galbo Estimation of rat muscle blood flow by microdialysis probes perfused with ethanol, [14C]ethanol, and 3H2O J Appl Physiol, March 1, 1999; 86(3): 1054 - 1061. [Abstract] [Full Text] [PDF]

				B. Stallknecht, J. Madsen, H. Galbo, and J. Bulow Evaluation of the microdialysis technique in the dog fat pad Am J Physiol Endocrinol Metab, March 1, 1999; 276(3): E588 - E595. [Abstract] [Full Text] [PDF]

				G. Radegran, H. Pilegaard, J. J. Nielsen, and J. Bangsbo Microdialysis ethanol removal reflects probe recovery rather than local blood flow in skeletal muscle J Appl Physiol, August 1, 1998; 85(2): 751 - 757. [Abstract] [Full Text] [PDF]