Journal of Applied Physiology, Vol 79, Issue 2 607-614, Copyright © 1995 by American Physiological Society

ARTICLES

Sarcolemmal disruption in reloaded atrophic skeletal muscle

C. E. Kasper
School of Nursing, University of California, Los Angeles 90095-6918, USA.

The purpose of this study was to determine whether reloading of atrophied skeletal muscle after 28 days of hind-limb unloading (HU) would produce significant sarcolemmal membrane disruption before frank necrosis. Soleus and plantaris muscles were atrophied by HU. Adult female Wistar rats (N = 13) were killed at 28 days of unloading and 4 and 7 days of reloading after HU. Rat serum albumin was used as a marker for muscle fiber disruption. Dark intracellular staining with horseradish peroxidase-conjugated anti-rat serum albumin antibody was interpreted as evidence of membrane rupture. There was a significantly different time course of disruption between plantaris and soleus muscles, with a negative correlation between cell size and occurrence of disruption. Fourteen percent of plantaris fibers were wounded after HU, peaking at day 4 of reloading (20% of cross-sectional area). Soleus demonstrated disruption only on reloading peaking in severity at day 7 (14% of fibers). It was demonstrated that sarcolemmal disruption due to atrophy and reloading does not always progress to necrosis and degeneration by the 7th day of recovery.

This article has been cited by other articles:

				J. J. Widrick, G. F. Maddalozzo, H. Hu, J. C. Herron, U. T. Iwaniec, and R. T. Turner Detrimental effects of reloading recovery on force, shortening velocity, and power of soleus muscles from hindlimb-unloaded rats Am J Physiol Regulatory Integrative Comp Physiol, November 1, 2008; 295(5): R1585 - R1592. [Abstract] [Full Text] [PDF]

				J. G. Tidball and M. Wehling-Henricks Macrophages promote muscle membrane repair and muscle fibre growth and regeneration during modified muscle loading in mice in vivo J. Physiol., January 1, 2007; 578(1): 327 - 336. [Abstract] [Full Text] [PDF]

				H. X. Nguyen, A. J. Lusis, and J. G. Tidball Null mutation of myeloperoxidase in mice prevents mechanical activation of neutrophil lysis of muscle cell membranes in vitro and in vivo J. Physiol., June 1, 2005; 565(2): 403 - 413. [Abstract] [Full Text] [PDF]

				H. X Nguyen and J. G Tidball Null mutation of gp91phox reduces muscle membrane lysis during muscle inflammation in mice J. Physiol., December 15, 2003; 553(3): 833 - 841. [Abstract] [Full Text] [PDF]

				H. X Nguyen and J. G Tidball Expression of a muscle-specific, nitric oxide synthase transgene prevents muscle membrane injury and reduces muscle inflammation during modified muscle use in mice J. Physiol., July 15, 2003; 550(2): 347 - 356. [Abstract] [Full Text] [PDF]

				P. E. Mozdziak, P. M. Pulvermacher, and E. Schultz Muscle regeneration during hindlimb unloading results in a reduction in muscle size after reloading J Appl Physiol, July 1, 2001; 91(1): 183 - 190. [Abstract] [Full Text] [PDF]

				K. Michael Relationship of Skeletal Muscle Atrophy to Functional Status: A Systematic Research Review Biol Res Nurs, October 1, 2000; 2(2): 117 - 131. [Abstract] [PDF]

				C. E. Kasper and L. P. Sarna Influence of Adjuvant Chemotherapy on Skeletal Muscle and Fatigue in Women with Breast Cancer Biol Res Nurs, October 1, 2000; 2(2): 133 - 139. [Abstract] [PDF]

				E. Zhu, A. S. Comtois, L. Fang, N. R. Comtois, and A. E. Grassino Influence of tension time on muscle fiber sarcolemmal injury in rat diaphragm J Appl Physiol, January 1, 2000; 88(1): 135 - 141. [Abstract] [Full Text] [PDF]

				P. E. Mozdziak, P. M. Pulvermacher, and E. Schultz Unloading of juvenile muscle results in a reduced muscle size 9 wk after reloading J Appl Physiol, January 1, 2000; 88(1): 158 - 164. [Abstract] [Full Text] [PDF]

				C. E. Kasper Recovery of Plantaris Muscle from Impaired Physical Mobility Biol Res Nurs, July 1, 1999; 1(1): 4 - 11. [Abstract] [PDF]

				C. E. Kasper Spatial Patterns of Atrophied Muscle Fibers during Exercised Recovery Biol Res Nurs, July 1, 1999; 1(1): 38 - 47. [Abstract] [PDF]

				M. S. F. Clarke, M. M. Bamman, and D. L. Feeback Bed rest decreases mechanically induced myofiber wounding and consequent wound-mediated FGF release J Appl Physiol, August 1, 1998; 85(2): 593 - 600. [Abstract] [Full Text] [PDF]