Journal of Applied Physiology
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J Appl Physiol 78: 205-211, 1995;
8750-7587/95 $5.00
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Journal of Applied Physiology, Vol 78, Issue 1 205-211, Copyright © 1995 by American Physiological Society

ARTICLES

Rapid tracer lactate influx into canine skeletal muscle

L. B. Gladden, R. E. Crawford, M. J. Webster and P. W. Watt
Department of Health and Human Performance, Auburn University, Alabama 36849.

This study evaluated the effects of various lactate transport inhibitors and competitors on rapid tracer lactate influx into the canine gastrocnemius-plantaris muscle (GP). GPs of 25 anesthetized dogs were perfused with red blood cell-free media in situ. At 0.9 mM lactate concentration ([La]), GP oxygen uptake (2.6 +/- 0.1 ml.kg-1.min-1) and net lactate output (-0.039 +/- 0.007 mmol.kg-1.min-1) were similar to values during blood perfusion. Rapid tracer lactate influx was inferred by a paired-tracer dilution method at nominal perfusate [La] values of 1, 5, 10, 25, and 50 mM. The maximal tracer influx rate (Umax) decreased significantly with each increase in unlabeled [La]. A saturation effect was suggested by the fact that percent inhibition of Umax began to reach a plateau at the higher unlabeled [La] values. The inhibition of Umax was 20.5 +/- 2.9% at 5 mM, 34.1 +/- 3.3% at 10 mM, 47.3 +/- 2.7% at 25 mM, and 56.1 +/- 2.8% at 50 mM [La]. Umax was also inhibited by various inhibitors/competitors of lactate transport as follows (% inhibition): 50 mM alpha-cyano-4-hydroxy-cinnamate (69.2 +/- 4.9%), 1.5 mM phloretin (25.4 +/- 5.5%), 0.1 mM 4,4'-diisothiocyanostilbene-2,2'-disulfonic acid (0.3 +/- 1.9%), 0.5 mM p-chloromercuribenzenesulfonic acid (72.9%), 0.5 mM furosemide (+ 2.8%), 25 mM pyruvate (52.4 +/- 2.9%), and 50 mM DL-lactate (50.2 +/- 4.0%). These experiments support the notion that lactate influx into canine skeletal muscle is a function of both a linear (possible diffusive) component and a Michaelis-Menten (carrier-mediated) component.


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[Abstract] [Full Text] [PDF]


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