Journal of Applied Physiology AJP: Cell Physiology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

J Appl Physiol 76: 1156-1165, 1994;
8750-7587/94 $5.00
This Article
Right arrow Full Text (PDF)
Right arrow Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Shephard, K. L.
Right arrow Articles by Rahmoune, H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Shephard, K. L.
Right arrow Articles by Rahmoune, H.

Journal of Applied Physiology, Vol 76, Issue 3 1156-1165, Copyright © 1994 by American Physiological Society

ARTICLES

Evaporation-induced changes in airway surface liquid on an isolated guinea pig trachea

K. L. Shephard and H. Rahmoune
Clore Laboratory for Life Sciences, University of Buckingham, United Kingdom.

An isolated preparation of the guinea pig trachea was developed. The trachea was exposed serosally to Krebs-Henseleit solution and mucosally to tidal airflow, designed to mimic conditions in vivo. The preparation establishes stable layers of airway surface liquid (ASL). Typical depth, transepithelial potential differences, and sodium activity are 200 microns, -3 mV, and 125 mM, respectively (approximate sodium concn 170 mM). When exposed to air with a vapor pressure deficit (VPD), evaporation of water occurs from ASL, ASL depth decreases, and the concentration of sodium ions in ASL increases. The water content of air passing over the trachea also increases. This measurement is compared with measurements of the change in volume of ASL, based on depth changes, to yield estimates of net water transport (NWT). Measurements of changes in the sodium content of ASL allow for the calculation of net sodium transport by the trachea. Evaporation rate, changes in the volume of ASL, NWT, and net sodium transport are all influenced by VPD. The results suggest that evaporation from ASL increases its sodium concentration (and osmotic pressure) and increases osmotically driven NWT to replace water lost. Evaporation-induced increases in the sodium concentration appear to be limited by enhanced sodium uptake at high VPD.


This article has been cited by other articles:


Home page
 Am. J. Respir. Crit. Care Med.Home page
A. N. FREED and M. S. DAVIS
Hyperventilation with Dry Air Increases Airway Surface Fluid Osmolality in Canine Peripheral Airways
Am. J. Respir. Crit. Care Med., April 1, 1999; 159(4): 1101 - 1107.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
D. X.-Y. Wu, C. Y. C. Lee, S. N. Uyekubo, H. K. Choi, S. J. Bastacky, and J. H. Widdicombe
Regulation of the depth of surface liquid in bovine trachea
Am J Physiol Lung Cell Mol Physiol, March 1, 1998; 274(3): L388 - L395.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
S. L. Winters and D. B. Yeates
Interaction between ion transporters and the mucociliary transport system in dog and baboon
J Appl Physiol, October 1, 1997; 83(4): 1348 - 1359.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
S. Duneclift, U. Wells, and J. Widdicombe
Estimation of thickness of airway surface liquid in ferret trachea in vitro
J Appl Physiol, September 1, 1997; 83(3): 761 - 767.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
J. Widdicombe
Airway and alveolar permeability and surface liquid thickness: theory
J Appl Physiol, January 1, 1997; 82(1): 3 - 12.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online