Journal of Applied Physiology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

J Appl Physiol 76: 33-38, 1994;
8750-7587/94 $5.00
This Article
Right arrow Full Text (PDF)
Right arrow Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Lefer, A. M.
Right arrow Articles by Ma, X. L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Lefer, A. M.
Right arrow Articles by Ma, X. L.

Journal of Applied Physiology, Vol 76, Issue 1 33-38, Copyright © 1994 by American Physiological Society

ARTICLES

PMN adherence to cat ischemic-reperfused mesenteric vascular endothelium under flow: role of P-selectin

A. M. Lefer and X. L. Ma
Department of Physiology, Jefferson Medical College, Thomas Jefferson University, Philadelphia, Pennsylvania 19107-6799.

We studied polymorphonuclear leukocyte (PMN) adherence to cat ischemic-reperfused mesenteric artery and vein endothelia under conditions of flow in vitro. Under physiological shear rates, only a few PMNs adhered to non-ischemic-reperfused arterial and venous endothelia (11 +/- 2 and 20 +/- 3 PMN/mm2, respectively). However, after 60 min of ischemia and 20 or 120 min of reperfusion, a significant increase in PMN adherence to arterial endothelium was observed. At 20 min of reperfusion, 44 +/- 4 PMN/mm2 adhered, and at 120 min postreperfusion, 63 +/- 12 PMN/mm2 adhered (P < 0.01 from control). Moreover, a greater degree of PMN adherence occurred on the venous than on the arterial endothelium. Thus, 159 +/- 10 and 198 +/- 12 PMN/mm2 adhered to mesenteric venous endothelium isolated after 20 and 120 min reperfusion, respectively (P < 0.01 vs. arteries). Furthermore, addition of PB 1.3 (20 micrograms/ml), a monoclonal antibody against P-selectin, 5 min before perfusion with PMNs significantly attenuated the increase in PMN adherence to both arterial and venous endothelia (P < 0.01). These results indicate that PMN-endothelial interaction also occurs in conduit vessels after ischemia and reperfusion, although a more profound PMN adherence occurs in veins.


This article has been cited by other articles:


Home page
 Biophys. JHome page
J. R. Hull, G. S. Tamura, and D. G. Castner
Structure and Reactivity of Adsorbed Fibronectin Films on Mica
Biophys. J., October 15, 2007; 93(8): 2852 - 2860.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
R. Hayward and A. M. Lefer
Time course of endothelial-neutrophil interaction in splanchnic artery ischemia-reperfusion
Am J Physiol Heart Circ Physiol, December 1, 1998; 275(6): H2080 - H2086.
[Abstract] [Full Text] [PDF]

Home page
 CirculationHome page
K. Fujise, B. M. Revelle, L. Stacy, E. L. Madison, E. T.H. Yeh, J. T. Willerson, and P. J. Beck
A Tissue Plasminogen Activator/P-Selectin Fusion Protein Is an Effective Thrombolytic Agent
Circulation, February 4, 1997; 95(3): 715 - 722.
[Abstract] [Full Text]

Home page
 Ann. Thorac. Surg.Home page
E. M. Boyle Jr, T. H. Pohlman, C. J. Cornejo, and E. D. Verrier
Endothelial Cell Injury in Cardiovascular Surgery: Ischemia-Reperfusion
Ann. Thorac. Surg., December 1, 1996; 62(6): 1868 - 1875.
[Abstract] [Full Text]

Home page
 Arterioscler. Thromb. Vasc. Bio.Home page
T. W. Gauthier, R. Scalia, T. Murohara, J.-p. Guo, and A. M. Lefer
Nitric Oxide Protects Against Leukocyte-Endothelium Interactions in the Early Stages of Hypercholesterolemia
Arterioscler. Thromb. Vasc. Biol., October 1, 1995; 15(10): 1652 - 1659.
[Abstract] [Full Text]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online