Journal of Applied Physiology
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J Appl Physiol 75: 1507-1512, 1993;
8750-7587/93 $5.00
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Journal of Applied Physiology, Vol 75, Issue 4 1507-1512, Copyright © 1993 by American Physiological Society


ARTICLES

Measuring intrasubject variability: use of the jacknife in doubly labeled water experiments

L. D. Broemeling and R. R. Wolfe
Metabolism Unit, Shriners Burns Institute, University of Texas Medical Branch, Galveston 77550.

The doubly labeled water technique measures energy expenditure; however, very little has appeared in the literature regarding estimation of the intrasubject variation. By use of a statistical resampling procedure called the jackknife, the standard deviation of the determination of energy expenditure in each subject is evaluated. Jackknife methods exploit the regression techniques that are already used with the doubly labeled water technique and are very easy to implement. Estimates of sample sizes for future experiments can easily be done with the jackknife. These formulas give the number of determinations of isotopic enrichment of hydrogen and oxygen over time that are needed to achieve a given degree of accuracy in estimating energy expenditure. An example with two human subjects illustrates the methodology of the jackknife.


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