| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Journal of Applied Physiology, Vol 74, Issue 5 2234-2241, Copyright © 1993 by American Physiological Society
ARTICLES |
J. M. Davis, W. N. Rosenfeld, R. J. Sanders and A. Gonenne
Department of Pediatrics (Neonatology), Winthrop University Hospital, State University of New York, School of Medicine, Stony Brook, Mineola 11501.
To determine if recombinant human Cu-Zn superoxide dismutase (rhSOD) would prevent acute lung injury caused by hyperoxia and barotrauma, 26 newborn piglets were studied. Ten piglets were hyperventilated (arterial PCO2 15-20 Torr) with 100% O2 for 48 h. A second group received identical treatment for 4 h (n = 2) or 48 h (n = 8) but was given 5 mg/kg of rhSOD intratracheally at time 0. Six piglets were normally ventilated (arterial PCO2 40-45 Torr) for 48 h with 21% O2. Pulmonary function and tracheal aspirates were examined at time 0 and at 24 and 48 h, and bronchoalveolar lavage was performed at 48 h. In piglets treated with hyperoxia and hyperventilation, lung compliance decreased 42%, and tracheal aspirates showed an increase in neutrophil chemotactic activity (32%), total cell counts (135%), elastase activity (93%), and albumin concentration (339%) over 48 h (P < 0.05). All variables were significantly lower in rhSOD-treated piglets and comparable to normoxic control values. Surfactant remained active in all groups. Immunohistochemistry demonstrated that at 48 h significant rhSOD was distributed homogeneously in terminal airways. Adding rhSOD to tracheal aspirates of hyperoxic hyperventilated piglets did not alter neutrophil chemotaxis, suggesting that rhSOD protected the lung by reducing the production of chemotactic mediators. Results indicate that acute lung injury caused by 48 h of hyperoxia and hyperventilation is significantly ameliorated by prophylactic intratracheal administration of rhSOD.
This article has been cited by other articles:
|
|
 |
|
  S. Lakshminrusimha, J. A. Russell, S. Wedgwood, S. F. Gugino, J. A. Kazzaz, J. M. Davis, and R. H. Steinhorn Superoxide Dismutase Improves Oxygenation and Reduces Oxidation in Neonatal Pulmonary Hypertension Am. J. Respir. Crit. Care Med., December 15, 2006; 174(12): 1370 - 1377. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. P. Kinsella, T. A. Parker, J. M. Davis, and S. H. Abman Superoxide Dismutase Improves Gas Exchange and Pulmonary Hemodynamics in Premature Lambs Am. J. Respir. Crit. Care Med., September 15, 2005; 172(6): 745 - 749. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H.-c. Koo, J. M. Davis, Y. Li, D. Hatzis, H. Opsimos, S. Pollack, M. S. Strayer, P. L. Ballard, and J. A. Kazzaz Effects of transgene expression of superoxide dismutase and glutathione peroxidase on pulmonary epithelial cell growth in hyperoxia Am J Physiol Lung Cell Mol Physiol, April 1, 2005; 288(4): L718 - L726. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Arita, A. Joseph, H.-C. Koo, Y. Li, T. A. Palaia, J. M. Davis, and J. A. Kazzaz Superoxide dismutase moderates basal and induced bacterial adherence and interleukin-8 expression in airway epithelial cells Am J Physiol Lung Cell Mol Physiol, December 1, 2004; 287(6): L1199 - L1206. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. M. Davis, R. B. Parad, T. Michele, E. Allred, A. Price, and W. Rosenfeld Pulmonary Outcome at 1 Year Corrected Age in Premature Infants Treated at Birth With Recombinant Human CuZn Superoxide Dismutase Pediatrics, March 1, 2003; 111(3): 469 - 476. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. H. JOBE and E. BANCALARI Bronchopulmonary Dysplasia Am. J. Respir. Crit. Care Med., June 1, 2001; 163(7): 1723 - 1729. [Full Text] [PDF]
|
|
|
|
 |
|
 A. M. Ilizarov, H.-C. Koo, J. A. Kazzaz, L. L. Mantell, Y. Li, R. Bhapat, S. Pollack, S. Horowitz, and J. M. Davis Overexpression of Manganese Superoxide Dismutase Protects Lung Epithelial Cells against Oxidant Injury Am. J. Respir. Cell Mol. Biol., April 1, 2001; 24(4): 436 - 441. [Abstract] [Full Text]
|
|
|
|
|
|
S. Das, S. Horowitz, C. G. Robbins, M. E. El-Sabban, N. Sahgal, and J. M. Davis Intracellular uptake of recombinant superoxide dismutase after intratracheal administration Am J Physiol Lung Cell Mol Physiol, May 1, 1998; 274(5): L673 - L677. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 I. Y. Haddad, B. Nieves-Cruz, and S. Matalon Inhibition of surfactant function by copper-zinc superoxide dismutase (CuZn-SOD) J Appl Physiol, November 1, 1997; 83(5): 1545 - 1550. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. E. Welty-Wolf, S. G. Simonson, Y.-C. T. Huang, S. P. Kantrow, M. S. Carraway, L.-Y. Chang, J. D. Crapo, and C. A. Piantadosi Aerosolized manganese SOD decreases hyperoxic pulmonary injury in primates. II. Morphometric analysis J Appl Physiol, August 1, 1997; 83(2): 559 - 568. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. M. Davis, W. N. Rosenfeld, S. E. Richter, R. Parad, I. H. Gewolb, A. R. Spitzer, W. A. Carlo, R. J. Couser, A. Price, E. Flaster, et al. Safety and Pharmacokinetics of Multiple Doses of Recombinant Human CuZn Superoxide Dismutase Administered Intratracheally to Premature Neonates With Respiratory Distress Syndrome Pediatrics, July 1, 1997; 100(1): 24 - 30. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Visit Other APS Journals Online |
