Journal of Applied Physiology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

J Appl Physiol 72: 1081-1089, 1992;
8750-7587/92 $5.00
This Article
Right arrow Full Text (PDF)
Right arrow Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Feng, N. H.
Right arrow Articles by Effros, R. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Feng, N. H.
Right arrow Articles by Effros, R. M.

Journal of Applied Physiology, Vol 72, Issue 3 1081-1089, Copyright © 1992 by American Physiological Society

ARTICLES

Solute exchange between the plasma and epithelial lining fluid of rat lungs

N. H. Feng, A. Hacker and R. M. Effros
Division of Pulmonary and Critical Care Medicine, Medical College of Wisconsin, Milwaukee 53226.

Although the transport of solutes from air spaces to plasma has been extensively studied, comparatively little information is available concerning solute equilibration between the plasma and the epithelial lining fluid (ELF) of air-filled lungs. In the present study, 11 lipophobic indicators varying in molecular mass between 22 and 80,000 Da were injected intravenously and/or intramuscularly into anesthetized rats in a manner designed to keep blood concentrations constant. The animals were killed by rapid lavage of their lungs at various intervals up to 120 min after the injections had been made. Indicator concentrations in the bronchoalveolar lavage (BAL) fluid and plasma were determined, and BAL-to-plasma concentration ratios were calculated for indicators that were injected (exogenous: [14C]urea, 22Na+, [3H]mannitol, 99mTc-diethylenetriaminepentaacetate (a chelate), 51Cr-(ethylene dinitrilo)tetraacetate (a chelate), 113mIn-transferrin, human albumin, and Evans blue-labeled rat albumin) and those that were already present from the plasma and ELF (unlabeled urea, rat albumin, and rat transferrin). Leakage of exogenous indicators in the blood into the BAL fluid was observed during the lavage procedure. Leakage of [14C]urea, 22Na+, and [3H]mannitol exceeded that of the heavier solute molecules. Diffusion of proteins and the labeled chelates into the ELF before lavage occurred at similar rates, suggesting vesicular transport. Use of rapidly diffusible solutes such as urea for determining dilution of ELF by BAL should be accompanied by intravascular injections of labeled solutes to correct for diffusion from the blood during lavage. Alternatively, labeled chelates or serum proteins can be used to estimate dilution of ELF by BAL. Interstitial sampling may be inevitable if the epithelium has been injured before lavage.


This article has been cited by other articles:


Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
D. Predescu, S. M. Vogel, and A. B. Malik
Functional and morphological studies of protein transcytosis in continuous endothelia
Am J Physiol Lung Cell Mol Physiol, November 1, 2004; 287(5): L895 - L901.
[Abstract] [Full Text] [PDF]

Home page
 Eur Respir JHome page
S. Bayat, K. Louchahi, B. Verdiere, D. Anglade, A. Rahoui, P-M. Sorin, M. Tod, O. Petitjean, F. Fraisse, and F.A. Grimbert
Comparison of 99mTc-DTPA and urea for measuring cefepime concentrations in epithelial lining fluid
Eur. Respir. J., July 1, 2004; 24(1): 150 - 156.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
S. BAYAT, A. MENAOUAR, D. ANGLADE, H. ETTINGER, A. FRANCOIS-JOUBERT, G. BENCHETRIT, and F. A. GRIMBERT
In Vivo Quantitation of Epithelial Lining Fluid in Dog Lung
Am. J. Respir. Crit. Care Med., December 1, 1998; 158(6): 1715 - 1723.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online