Journal of Applied Physiology AJP: Cell Physiology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

J Appl Physiol 71: 1751-1757, 1991;
8750-7587/91 $5.00
This Article
Right arrow Full Text (PDF)
Right arrow Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Tsan, M. F.
Right arrow Articles by White, J. E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Tsan, M. F.
Right arrow Articles by White, J. E.

Journal of Applied Physiology, Vol 71, Issue 5 1751-1757, Copyright © 1991 by American Physiological Society

ARTICLES

Erythrocyte insufflation-induced protection against oxygen toxicity: role of cytokines

M. F. Tsan, D. Lawrence and J. E. White
Department of Veterans Affairs Medical Center, Albany, New York.

We studied the pulmonary response of adult rats to erythrocyte (RBC) and RBC lysate insufflation to define the mechanism of RBC insufflation-induced protection against oxygen toxicity. Tracheal insufflation of 1 ml RBC (75%) or RBC lysate induced an intense pulmonary inflammatory response. Within 24 h of oxygen exposure, greater than 95% of insufflated RBCs was hemolyzed. The cell-free fraction of alveolar lavage fluids from RBC- or RBC lysate-insufflated rats had similar capacity in protecting endothelial cells against H2O2 cytotoxicity. However, RBC insufflation but not RBC lysate insufflation, protected rats against oxygen toxicity. There was marked erythrophagocytosis by alveolar macrophages of RBC-insufflated rats. Insufflation of RBCs, but not RBC lysate, resulted in production of tumor necrosis factor and interleukin 1, which could be recovered by bronchoalveolar lavages. When rats were insufflated with a combination of RBC lysate and cytokines at dosages within the range of cytokine levels achievable in alveolar lavage fluids by RBC insufflation, they became tolerant to oxygen. These results suggest that endogenously produced tumor necrosis factor and interleukin-1 as a result of RBC insufflation may play an important role in RBC insufflation-induced oxygen tolerance.


This article has been cited by other articles:


Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
C. E. Clayton, M. S. Carraway, H. B. Suliman, E. D. Thalmann, K. N. Thalmann, D. E. Schmechel, and C. A. Piantadosi
Inhaled carbon monoxide and hyperoxic lung injury in rats
Am J Physiol Lung Cell Mol Physiol, October 1, 2001; 281(4): L949 - L957.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
M.-F. Tsan, R. N. Clark, S. M. Goyert, and J. E. White
Induction of TNF-{alpha} and MnSOD by endotoxin: role of membrane CD14 and Toll-like receptor-4
Am J Physiol Cell Physiol, June 1, 2001; 280(6): C1422 - C1430.
[Abstract] [Full Text] [PDF]

Home page
 Innate ImmunityHome page
J. E. White and M.-F. Tsan
Induction of TNF-{alpha} and MnSOD by endotoxin: effect of E5531, a synthetic non-toxic lipid A analog
Innate Immunity, August 1, 2000; 6(4): 329 - 335.
[Abstract] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
L. Tian, J. E. White, H.-Y. Lin, V. S. Haran, J. Sacco, G. Chikkappa, F. B. Davis, P. J. Davis, and M.-F. Tsan
Induction of Mn SOD in human monocytes without inflammatory cytokine production by a mutant endotoxin
Am J Physiol Cell Physiol, September 1, 1998; 275(3): C740 - C747.
[Abstract] [Full Text] [PDF]

Home page
 Innate ImmunityHome page
J.T. Berg, G. Tang, and M.F. Tsan
Protection against oxygen toxicity by lipid A: role of Mn superoxide dismutase
Innate Immunity, June 1, 1994; 1(2): 108 - 113.
[Abstract] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online