Journal of Applied Physiology, Vol 71, Issue 4 1225-1230, Copyright © 1991 by American Physiological Society

ARTICLES

Cytochrome c protein synthesis rate in rat skeletal muscle

F. W. Booth
Department of Physiology and Cell Biology, University of Texas Medical School, Houston 77225.

Endurance training is associated with increases in mitochondrial density, of which cytochrome c protein is an index. Increases in the synthesis rates of cytochrome c protein in skeletal muscle during endurance training have been inferred (Biochem. Biophys. Res. Commun. 66: 173, 1975; J. Biol. Chem. 252: 416, 1977). One purpose of the present study was to test these indirect approximations with direct measurements of the synthesis rates of cytochrome c protein in skeletal muscles postexercise. No change in the fractional synthesis rate of cytochrome c was detected in the red quadriceps muscle of rats either 2-7 h after a 104-min run on a motor-driven treadmill or 17-22 h after the final bout of 4 days of running 100 min/day. If the 16% increase in cytochrome c protein concentration in the red quadriceps muscle on the 5th day of training is used to calculate the nanomoles of cytochrome c synthesized per gram of wet muscle weight, the normalized rate of cytochrome c protein synthesis is increased 29% on the 5th day of training. The observation of no significant alteration in cytochrome c mRNA in the red quadriceps muscle of rats during the 1st wk of training implies that the initial increase in the synthesis rate of cytochrome c protein normalized per unit of muscle mass during treadmill training is likely to occur at a translational or posttranslational step. These results suggest that the control of increased cytochrome c expression in skeletal muscle during exercise training involves a complex mechanism.

This article has been cited by other articles:

				P. M. Garcia-Roves, J. Huss, and J. O. Holloszy Role of calcineurin in exercise-induced mitochondrial biogenesis Am J Physiol Endocrinol Metab, June 1, 2006; 290(6): E1172 - E1179. [Abstract] [Full Text] [PDF]

				P. J. LeBlanc, S. J. Peters, R. J. Tunstall, D. Cameron-Smith, and G. J. F. Heigenhauser Effects of aerobic training on pyruvate dehydrogenase and pyruvate dehydrogenase kinase in human skeletal muscle J. Physiol., June 1, 2004; 557(2): 559 - 570. [Abstract] [Full Text] [PDF]

				F. Sasarman, G. Karpati, and E. A. Shoubridge Nuclear genetic control of mitochondrial translation in skeletal muscle revealed in patients with mitochondrial myopathy Hum. Mol. Genet., July 1, 2002; 11(14): 1669 - 1681. [Abstract] [Full Text] [PDF]

				Y. Burelle and P. W. Hochachka Endurance training induces muscle-specific changes in mitochondrial function in skinned muscle fibers J Appl Physiol, June 1, 2002; 92(6): 2429 - 2438. [Abstract] [Full Text] [PDF]

				R. Bergeron, J. M. Ren, K. S. Cadman, I. K. Moore, P. Perret, M. Pypaert, L. H. Young, C. F. Semenkovich, and G. I. Shulman Chronic activation of AMP kinase results in NRF-1 activation and mitochondrial biogenesis Am J Physiol Endocrinol Metab, December 1, 2001; 281(6): E1340 - E1346. [Abstract] [Full Text] [PDF]

				D. Xiao, C. A. Ducsay, and L. Zhang Chronic Hypoxia and Developmental Regulation of Cytochrome C Expression in Rats Reproductive Sciences, September 1, 2000; 7(5): 279 - 283. [Abstract] [PDF]