Journal of Applied Physiology, Vol 71, Issue 3 1106-1111, Copyright © 1991 by American Physiological Society

ARTICLES

Lung to blood passage of different-sized molecules during lung inflammation in the rat

H. G. Folkesson, B. R. Westrom, S. G. Pierzynowski and B. W. Karlsson
Department of Animal Physiology, University of Lund, Sweden.

The passage of different-sized marker molecules over the lower respiratory tract into the blood circulation during pulmonary inflammation induced by dextran, endotoxin [i.e., lipopolysaccharide from Escherichia coli (LPS)], or ferritin was assessed in the rat. Bovine immunoglobulin G (BIgG, mol wt = 150,000 Da), bovine serum albumin (BSA, mol wt = 67,000 Da), and the nonapeptide 1-deaminocysteine-8-D-arginine vasopressin (dDAVP, mol wt = 1,067 Da) were used as permeability markers after intratracheal instillation. The pathophysiological indexes of a proceeding lung inflammation were increased total cell number, changed leukocyte proportions and increased total protein content obtained in bronchoalveolar lavage, and lung edema formation shown as an increased lung wet-dry weight difference. Intratracheal instillation of dextran induced a moderate neutrophil invasion into the lungs but had no effect on the passage of the different markers over the lungs (BIgG 1.8 +/- 0.6%, BSA 3.5 +/- 1.2%, dDAVP 26.1 +/- 20.7%) compared with control rats instilled with the markers alone (1.8 +/- 0.4%, 4.1 +/- 1.3%, 20.0 +/- 3.8%, respectively). Endotoxin administration resulted in markedly higher lavage cell counts and lung edema concomitantly with an increased lung passage of the markers (3.2 +/- 0.9%, 22.0 +/- 6.1%, 33.3 +/- 12.0%, respectively; P less than 0.01-P less than 0.001). The highest marker passage was obtained when the inflammation was most severe, i.e., after ferritin administration (17.6 +/- 2.3%, 60.0 +/- 6.7%, 41.6 +/- 6.9%, respectively; P less than 0.001), which resulted in markedly elevated lavage cell numbers and protein content as well as edema formation.(ABSTRACT TRUNCATED AT 250 WORDS)

This article has been cited by other articles:

				K.-J. Kim and A. B. Malik Protein transport across the lung epithelial barrier Am J Physiol Lung Cell Mol Physiol, February 1, 2003; 284(2): L247 - L259. [Abstract] [Full Text] [PDF]

				A. J. Simpson, W. A. H. Wallace, M. E. Marsden, J. R. W. Govan, D. J. Porteous, C. Haslett, and J.-M. Sallenave Adenoviral Augmentation of Elafin Protects the Lung Against Acute Injury Mediated by Activated Neutrophils and Bacterial Infection J. Immunol., August 1, 2001; 167(3): 1778 - 1786. [Abstract] [Full Text] [PDF]

				Y. KITAMURA, S. HASHIMOTO, N. MIZUTA, A. KOBAYASHI, K. KOOGUCHI, I. FUJIWARA, and H. NAKAJIMA Fas/FasL-dependent Apoptosis of Alveolar Cells after Lipopolysaccharide-induced Lung Injury in Mice Am. J. Respir. Crit. Care Med., March 1, 2001; 163(3): 762 - 769. [Abstract] [Full Text] [PDF]

				S. W. STITES, M. W. PLAUTZ, K. BAILEY, A. R. O'BRIEN-LADNER, and L. J. WESSELIUS Increased Concentrations of Iron and Isoferritins in the Lower Respiratory Tract of Patients with Stable Cystic Fibrosis Am. J. Respir. Crit. Care Med., September 1, 1999; 160(3): 796 - 801. [Abstract] [Full Text]

				C. HERMANS and A. BERNARD Lung Epithelium-specific Proteins . Characteristics and Potential Applications as Markers Am. J. Respir. Crit. Care Med., February 1, 1999; 159(2): 646 - 678. [Full Text]

				B. P. Guery, S. Nelson, N. Viget, P. Fialdes, W. R. Summer, E. Dobard, G. Beaucaire, and C. M. Mason Fluorescein-labeled dextran concentration is increased in BAL fluid after ANTU-induced edema in rats J Appl Physiol, September 1, 1998; 85(3): 842 - 848. [Abstract] [Full Text] [PDF]

				W. Taljanski, S. G. Pierzynowski, P. D. P. Lundin, B. R. Weström, S. Eirefelt, J. Podlesny, M. Dahlbäck, H. Siwinska-Golebiowska, and B. W. Karlsson Pulmonary Delivery of Intratracheally Instilled and Aerosolized Cyclosporine A to Young and Adult Rats Drug Metab. Dispos., August 1, 1997; 25(8): 917 - 920. [Abstract] [Full Text]

				H. G. FOLKESSON, B. R. WESTRÖM, and B. W. KARLSSON Effects of Systemic and Local Immunization on Alveolar Epithelial Permeability to Protein in the Rat Am. J. Respir. Crit. Care Med., January 1, 1997; 157(1): 324 - 327. [Abstract] [Full Text]