Journal of Applied Physiology Fuel your research with LabChart
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

J Appl Physiol 70: 63-69, 1991;
8750-7587/91 $5.00
This Article
Right arrow Full Text (PDF)
Right arrow Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Ballard, S. T.
Right arrow Articles by Gatzy, J. T.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Ballard, S. T.
Right arrow Articles by Gatzy, J. T.

Journal of Applied Physiology, Vol 70, Issue 1 63-69, Copyright © 1991 by American Physiological Society

ARTICLES

Alveolar transepithelial potential difference and ion transport in adult rat lung

S. T. Ballard and J. T. Gatzy
Department of Pharmacology, School of Medicine, University of North Carolina, Chapel Hill 27514.

The complex morphology of the mammalian lung complicates characterization of solute transport across the intact alveolar epithelium. We impaled the subpleural alveolar epithelium with microelectrodes and measured the transepithelial potential difference (PD) of the liquid-filled vascular-perfused left lobe of the rat lung. When the air space was filled entirely with Krebs-Ringer-bicarbonate, the PD was 4.7 mV (lumen negative). The PD was not affected significantly by agents that modify either Na+ or Cl- transport, but replacement of luminal Cl- with gluconate resulted in a fourfold hyperpolarization, a response also noted for large airways. When the airways were blocked by an immiscible nonconducting fluorocarbon, basal PD was not different from unblocked lobes (4.0 mV) but was inhibited 73% by luminal amiloride. Cl(-)-free Krebs-Ringer-bicarbonate blocked in the alveoli with fluorocarbon did not induce hyperpolarization. This result suggests that 1) Cl- permselectivity of the alveolar epithelium is less than that of large airway epithelium and 2) airway PD dominates the voltage across the liquid-filled lung, even when measurements are made from alveoli. When airways are blocked by fluorocarbon, the PD across the alveolar epithelium is largely dependent on Na+ flow through a path with amiloride-sensitive channels.


This article has been cited by other articles:


Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
L. G. Rochelle, D. C. Li, H. Ye, E. Lee, C. R. Talbot, and R. C. Boucher
Distribution of ion transport mRNAs throughout murine nose and lung
Am J Physiol Lung Cell Mol Physiol, July 1, 2000; 279(1): L14 - L24.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
S. M. O'Grady, X. Jiang, and D. H. Ingbar
Cl-channel activation is necessary for stimulation of Na transport in adult alveolar epithelial cells
Am J Physiol Lung Cell Mol Physiol, February 1, 2000; 278(2): L239 - L244.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
T. E. DeCoursey
Hypothesis: do voltage-gated H+ channels in alveolar epithelial cells contribute to CO2 elimination by the lung?
Am J Physiol Cell Physiol, January 1, 2000; 278(1): C1 - C10.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
X. Jiang, D. H. Ingbar, and S. M. O'Grady
Adrenergic stimulation of Na+ transport across alveolar epithelial cells involves activation of apical Cl- channels
Am J Physiol Cell Physiol, December 1, 1998; 275(6): C1610 - C1620.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online